These were then inoculated on Blood, MacConkey and Chocolate agar
and incubated at 37degC for 24-48 hours.
69%) of patients were positive by Culture, in spite of using Brucella chocolate agar
with selective agents like (Polymyxin B, Amphotericin B and Vancomycin).
Media that were adequate for culturing bacteria before performing the rapid polymyxin NP test were Luria Bertani agar, Mueller-Hinton agar, Columbia agar + 5% sheep blood, chocolate agar
, UriSelect 4 agar, and eosin methylene blue agar.
It was also inoculated into blood agar, chocolate agar
, and brain heart infusion (BHI) agar and incubated at 37[degrees]C.
The culture media used included nutrient agar, MacConkey agar and chocolate agar
The aspirate was inoculated on to blood and chocolate agar
(CO2 incubation at 37[degrees]C) and Fastidious Anaerobe Agar (anaerobic incubation at 37[degrees]C).
The specimen was inoculated onto 5% sheep blood agar, chocolate agar
, MacConkey agar and anaerobic blood agar for bacterial isolation.
661 patients met the criteria; their age, sex, levels of CSF glucose, protein, white blood cell (wbc) count and reports of CSF culture in chocolate agar
media at admission as well as their prognosis on 15 day follow up based on extended Glasgow outcome score (GOS-E) were documented.
Two sets of blood agar and chocolate agar
plates were prepared and were tested for the growth of Acanthamoeba with autoclaved and live E.
These organisms are facultative anaerobic and grow on routine laboratory media such as blood (BAP), chocolate agar
plate (CAP), colistinnalidixic acid (CAN), and phenyl ethyl alcohol (PEA) agars.
1% cysteine (TBS-C) or peptone-yeast extract-glucose (PYG) at 37 [degrees]C with shaking, or on chocolate agar
The pus was plated on chocolate agar
and MacConkey's agar.
The identity of the organism was confirmed by specific characteristics after culture on chocolate agar
supplemented with PolyVitex plus colistin, vancomycin, amphotericin B, and trimethoprim antibiotics (bioMerieux SA).
01-ml calibrated loop and inoculated into a Columbia agar base supplemented with 5% sheep's blood, chocolate agar
, eosin-methylene-blue agar, and Chapman agar (for aerobic and facultative bacteria) and onto a Brucella agar base supplemented with 5% sheep's blood, vitamin [K.
Yersinia pestis grows well both on 5% sheep blood agar and on chocolate agar
, as well as in brain heart infusion broth and most commercial blood culture media.