Key words: Chicken anemia virus, gumboro, reovirus, broilers chicken, ELISA.
Detection of chicken anemia virus in gonads and in the progeny of broiler breeder hens with high neutralizaing antibody titers.
Serial propagation and purification of chicken anemia agent in MDCC - MSB1 cell line.
To determine disease seroprevalence, commercial enzyme-linked immunosorbent assays (ELISAs; Idexx Inc, West Brook, ME, USA) were performed for infectious bursal disease (IBD, gumboro disease), avian encephalomyelitis virus (AE, Picornaviridae), chicken anemia virus (CAV, Circoviridae), Newcastle disease virus (NDV, Paramyxoviridae), avian influenza (Orthomyxoviridae, type A), avian infectious bronchitis (IBV, coronavirus), Mycoplasma gallisepticum, and Mycoplasma synoviae (University of Georgia Poultry Diagnostic and Research Center, Athens, GA, USA).
For chicken anemia virus, results were considered positive when the optical density was <1.
In 90 (90%) birds, antibodies were detected for chicken anemia virus.
Inability of so-called chicken anemia agent (CAA) infections to be diagnosed by anemia and hematopoietic organ atrophy alone.
Ultrastructure of bone marrow in chicks inoculated with chicken anemia agent (MSB1-TK5803 strain).
Persistence and vertical transmission of chicken anemia agent in experimentally infected laying hens.