The detection method in microarray chips is based on microscopy analysis via the use of fluorophore or chemoluminescence
labels that determine abundance of nucleic acid sequences.
In Abstacts of the 11th International Symposium on Bioluminescence and Chemoluminescence
In their recent paper, Anderson & Beaven (2005) described a chemoluminescence
method measuring the oxidative burst in lobsters after activation with Phorbol myristate acetate (PMA).
Scientists aren't sure why we glow, but they suspect it's because of chemoluminescence
, or light-producing chemical reactions similar to those in fireflies.
1%, and 0%) in 2x SSPE, the membranes were incubated for 2 minutes with the enhanced chemoluminescence
(ECL) detection reagents (Amersham Life Science, s`Hertogenbosch, the Netherlands), and then were exposed to an ECL hyperfilm (Amersham Life Science) for 30 minutes and overnight to visualize the bound probe.
Alkaline phosphatase-conjugated goat antimouse IgG (Dianova) was used at a dilution of 1:20 000 for the detection of specific antibody binding with a chemoluminescence
kit (Tropix) and an exposure time of 10 min.
produced by DNA-RNA hybrids is measured in the Gen-Probe luminometer.