The His-D2EDIII DNA fragment is stably maintained within the PRSV genome: pVD2EDIII second passage was performed using the cell sap
prepared from either the fresh or frozen infected leaf samples from the first passage.
Leaves were frozen and pressed with a hydraulic press to collect cell sap
for the analysis of solute concentration and osmotic potential.
However, there is a major problem with this theory, Yoshida points out: Plant cell sap
usually is weakly acidic or at most neutral.
Papyrus and the Adhesive Properties of its Cell Sap
in relation to Papermaking', J.
Chloride concentration was the highest in leaf cell sap
with median values of 150 ppm, while it was the lowest for CaSO4 (30 mM) in wheat leaf at booting stage, with a median value of 125 ppm.
Rarely is the loss of liquid material of major harm to the plant, as it simply increases production of cell sap