It has been stated that the initial increase of the cell number is due to the cell fusion
process during the initial response of cell to the toxicant, which can occur in apoptosis process (Xing et al.
Modern biotechnology entails the use of cell fusion
techniques, bio-informatics, genetic engineering, structure based molecular design and recombinant DNA technology, and hybridoma technology.
Two methods of cell fusion
have been used in the past by various groups researching MRT.
One of the mechanisms being studied is reprogramming through cell fusion
The study demonstrated that the intrinsic flexibility of the attachment protein is a necessary condition to initiate the cell fusion
The 22 chapters presented here by Pells (gene function and development, Roslin Institute, UK) describe methods of nuclear reprogramming including isolation of stromal cells from human adipose tissue, cell fusion
, polyethylene glycol-mediated cell fusion
, epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells, modulation of cell fate using nuclear and cytoplasmic extracts, using immunofluorescence to observe methylation changes in mammalian preimplantation embryos, staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens, and methylation-sensitive polymerase chain reaction.
Progress is being made in understanding transcription regulation and cell fusion
Genzyme has the option to obtain an exclusive license to the dendritic/cancer cell fusion
technology from Dana Farber.
A novel process involving what appears to be exchange of plasmids by partial cell fusion
has been observed in Halobacterium volcanii (16), and exchange of genetic markers has also been reported in Sulfolobus acidocaldarius (17).
Objective: Cell fusion
is critical for fertilization and development, for instance underlying muscle or bone formation.
But we also have provided the first evidence that BMDCs can contribute simultaneously to different encephalic areas through different mechanisms of plasticity: cell fusion
for Purkinje cells, which are among the largest and most elaborately dendritic neurons in the human brain, and differentiation for olfactory bulb interneurons.
Our study shows that transplanted bone marrow cells fuse not only with bone marrow cells of the recipient, but with non-hematopoietic cells, suggesting that if we can understand the process of cell fusion
better, we may be able to target certain organ injuries with the patient's own bone marrow cells and repair the tissues," said Dr.