are normal, and they may have some benefits," Lieberman said.
In the present study, the calluses
obtained from shoot tip, 1st and 2nd nodal explants in the MS medium fortify with TDZ (0.05-1.00 mg/L) were selected and transferred to the MS nutrient medium without plant growth regulator (MSO) and to MS medium containing 0.10 mg/L BAP and 0.10 mg/L GA3 (Table 2), which is to achieve multiple shoot regeneration by sub-culturing callus.
Two distinct experiments were carried out to induce calluses
Then obtained calluses
(n = 135) were divided into control group (medium without NPs) and three experimental groups, where MS medium was supplemented by different [Fe.sub.3][O.sub.4] NPs concentrations (0.5 mg/ 1, 1 mg/l, and 1.5 mg/l) and was left on medium for 4 weeks.
Subculturing was done of the calluses
to fresh medium until the shoots emerged, which were then transferred to the MS medium with decreased sucrose concentration (30g [L.sup.-1]) in the absence of growth regulators for the shoots to develop.
usitatissimum "Lirina" was used for calluses
formation and in vitro cultures were established according to Kokina et al.
Extracts from stalk, pedicel, bloom flower (flower stage 4), bud (flower stage 1), and calluses
exhibited high antioxidant effects.
Medical attention usually isn't needed for corns and calluses
unless you have a health condition that impacts blood flow to the feet (for example, diabetes).
In general, one can consider that the callus was more intense in the month of July, when there was 100% of layers with calluses
and these exhibited higher dry mass when compared to the others, regardless of the substrate evaluated.
Although, seemingly a trivial condition, it has been shown that older people, in particular, who have plantar calluses
have difficulty with walking and using stairs (3).
Your feet bear the brunt of your weight, and they are susceptible to everything from calluses
to athlete's foot, so why don't you pamper them every once in a while?
It showed a good callus diameter and as well as loose calluses
on medium (Graph 7).
We think that the cause of this at the initial period of regeneration is presence of massive parosteal and periosteal calluses
closely attached to the surrounding soft tissue with good vascularization and incomplete development of endosteal callus.
The content of total soluble sugar has increased during the development of calluses
, indicating the absorption from the medium and synthesis of new carbohydrates resulting in growth with variation in the concentration of reducing sugars.