Some studies applied BODIPY iodoacetamide compounds produced by this company that are now discontinued (i.e., BODIPY[R] 530/550 IA, BODIPY[R] FL IA and BODIPY[R] TMR CADAVERINE
was added aseptically at levels of 0, 40, 80, and 120 mM to the sterilized BTJA medium.
Levels of cadaverine
, putrescine, spermidine, and spermine in the crown nodules of Chippewa and Hardin also were evaluated from 10 to 59 DAE.
A lot of study has previously reported on determination of fish freshness by physical observation on eyes, gills, and skin [2, 3], microbial counts [3, 4], presence of biogenic amines such as histamine, cadaverine
, and putrescine [5-7], determination of trimethylamineoxide (TMAO) [8, 9], and lipid oxidation .
Biogenic amines, such as histamine (His), cadaverine
, putrescine, spermine, and spermidine, are breakdown products that are produced during decarboxylation of amino acid catabolism.
In Escherichia coli and some other coliforms, lysine decarboxylase is encoded by cadA which participates in the decarboxylation of lysine and synthesis of cadaverine
These authors did not detect histamine, a peripheral vasodilator, and the vasoconstrictors tyramine, tryptamine and phenylethylamine, as well as cadaverine
in the fruits.
Polyamines associated with putrefaction, e.g., cadaverine
, putrescine and agmatine, were also detected in increasing concentrations after 7 d.
For the comparison of different PSAO samples 0.12 mM cadaverine
solution was used as the standard.
The bacteria that cause the condition produce organic byproducts with such telling, if unsavory, names as putrescine and cadaverine
. The first also emanates from rotting fish, the second from corpses.
The eleven biogenic amines studied were ethylamine (ETA), methylamine (MEA), histamine (HIS), serotonin (SER), spermine (SPM), spermidine (SPD), agmatine (AGM), putrescine (PUT), [beta]-phenylethylamine ([beta]-PEA), cadaverine
(CAD), and tyramine (TYM) all of which were supplied by Supelco, Bellefonte, PA, USA, as well as the derivatizing agent dansyl chloride and the internal standard 1,7-diaminoheptane (IS).
Methylamine, putrescine and cadaverine
were only detected in exclusively SM and 7:3 ratios.
The surprise, Decker says, is that the known antioxidants that they expected to be protective-compounds with such unappetizing names as putrescine, cadaverine
, spermine, and spermadine-accounted for only a small fraction of the effect.
The use of a bacterial starter culture in the manufacture of mould-ripened wild boar salami resulted in significantly lower peroxide values, lower TBARS concentrations, and lower amounts of biogenic amines, namely, histamine, cadaverine
, and putrescine, associated with better sensory evaluation scores.
The assay mixture (3.8 mL) contained 3 mL of phosphate buffer (0.2 M, pH 7.2), 0.1 mL (0.004%) of horseradish peroxidase solution (Sigma Chemicals), 0.1 mL of o-dianisidine-methanol solution (0.5% of o-dianisidine [Sigma Chemicals] in methanol), 0.5 mL of plasma, and 0.1 mL of substrate solution (0.175% of cadaverine
dihydrochloride, Sigma Chemicals).