If the primers flanking the target region of interest start at nucleotide number 500 at the 5[feet] site and end at nucleotide 800 at the 3[feet] site, the predicted PCR cDNA
signal will be 300 bp [ILLUSTRATION FOR FIGURE 1 OMITTED].
Not surprisingly, the corporations frequently wondered were what they were getting from CDNA
At least two biotechnology companies are mass-sequencing cDNA
and applying for patents on some of those findings.
The sequence of Wild Argali ISG15 cDNA
and the inferred amino acids (Figure 4) were submitted to GenBank as the accession no.
was generated using PrimeScript(r) RT reagent Kit (Takara, Japan) according to the manufacturer's recommendations and then the second-strand cDNA
The sequence of Wild Argali SPLUNC1 cDNA
and the inferred amino acids was shown in Figure 2B.
First strand cDNA
was synthesized using a PrimeScriptTM RT Reagent Kit (TaKaRa).
library construction: Cells were harvested and total RNA was extracted using Trizol reagent (Invitrogen Carlsbad CA USA) when they were in the period of passage 3.
In order to sub-clone the cDNA
in pCMV6Neo expression vector, KpnI and HindIII restriction sites were embedded at the proximity of start codon of forward primer and 3 end of stop codon in reverse primer, respectively.
was synthesized from 1 g RNA using PrimeScript(R) RT reagent Kit With gDNA Eraser (TaKaRa Japan).
The supply and distribution agreement allows Horizon to offer gene modulation technologies, including stable shRNA knockdown, cDNA
overexpression systems, as well as editing of endogenous genes through rAAV, ZFN and CRISPR platforms.
M2 EQUITYBITES-February 10, 2014-Bioline reports worldwide market launch of high performance SensiFAST cDNA
synthesis kit, expanding its portfolio of reliable, highly specialized molecular biology reagents
a leading viral gene-delivery systems provider, today announces the launch of its world's largest collection of premade human full-length cDNA
ORF and miRNA adenoviruses.
is generated from the processed RNA transcripts and contains largely just the protein-coding portions of the genetic information.
The library of samples includes genomic DNA preparations, tissue specific PCR-ready first strand cDNAs
, universal cDNA
and 96- and 384-well cDNA