The relative weight of bursa of Fabricius
was increased by supplementation with various levels of 1,3-DAG (quadratic, P<0.05).
Functionally, the lymphoid tissue has been classified into the primary lymphoid tissue, such as the thymus and bursa of Fabricius
, and the secondary lymphoid tissue, such as the spleen (figure 1) (MADEJ et al., 2015; Sun et al., 2016; IFRAH et al., 2017).
For necropsy, the tissues of brain, trachea, caecal tonsil, liver, spleen, bursa of Fabricius
, proventriculus, and thymus were fixed in 10% buffered formalin.
Acute, marked thymic lymphocytic apoptosis, splenic necrosis, and lymphoid depletion in the bursa of Fabricius
were also present in both birds; immunohistochemical staining for WFBV was positive in the thymus and spleen but not in the bursa of Fabricius
Relative Weight of the Bursa of Fabricius
and Bursometry: Five birds from each treatment groups were randomly weighted and slaughtered on 7, 14, 21, 28 and 35 day of age.
Then bursa of Fabricius
was separated from adhering tissues and all samples were frozen in liquid nitrogen and stored at -80[degrees]C.
Five days after infection (dpi), three goslings from each group were euthanized, and eight tissues (bursa of Fabricius
, cecum, cecal tonsil, Harderian gland, lung, small intestine, spleen, and thymus) were collected immediately for RNA isolation and cDNA synthesis.
Source of First-Generation Bursa of Fabricius
ATTI."ConvPotalAviare, pp 77-83 (1975). Ezeokoli, C.D.,Ityondo, E.A., Nwannenna, A.I., Umoh, J.U.,"Immunosuppression and histopathological changes in the bursa of Fabricius
associated with infectious bursal disease vaccination in chicken."Comp Immunology Microbiology of Infectious Diseases13:181-8 (1990). Ali, A.S., Abdalla, M.O, and Mohammed,"Interaction between Newcastle disease and infectious bursal disease vaccines commonly used in Sudan." International Journal of Poultry Sciences, 3:300-304 (2004).Nakamura, Y., Otaki, T., and Nunoya," Immunosuppressive Effect of a Highly Virulent Infectious Bursal Disease Virus Isolated in Japan." Aviam, n Diseases, 36:891-896(1992
The blood immunoglobulin, bursa of Fabricius
, thymus, and spleen weight were significantly reduced when broilers were exposed to EHS.
The bursa of Fabricius
is the main target organ for IBDV replication .
This atrophy in the Bursa of Fabricius
most likely leads to immunosuppression (Lukert and Saif, 2003), which could affect negatively the feed conversion (Sharma et al., 2000).
We used two measures of immune response in avian hosts, the size of the bursa of Fabricius
and the spleen in our comparative studies.
Lymphoid organs (thymus, spleen and bursa of fabricius
) were removed and weighed separately.
The weight of breast, gizzard (empty), proventriculus (empty), pancreas, liver, bursa of fabricius
, spleen, and abdominal fat of each slaughtered bird were recorded and considered as g of 100 g of live weight.