Zygosity testing results determined by ratio analysis for both plasma (cfDNA)-and buffy coat
(gDNA)-extracted DNA samples of human whole blood samples.
Quantitative Buffy Coat
Technique (QBC): Quantitative buffy coat
technique (BD Diagnostics) was employed for the detection of malarial parasites in blood.
Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat
analysis and in vitro culture of Borrelia crocidurae.
Usefullness of Quantitive Buffy Coat
Blood Parasite detection system in diagnosis of malaria.
A typical whole blood sample from a healthy individual contains 4-7 X [10.sup.6] leukocytes/mL of blood, (9) so this cell input was well within the range applicable to buffy coat
samples taken from 2-5 mL of whole blood.
(13) The hematoparasites and red blood cells infected by them come to rest in the buffy coat
area or slightly below it due to their density being lower than the non-infected red cells.
This is supported by our data showing that the hCG interference was not observed in the sample spiked with heat-treated buffy coat
, suggesting a heat labile interference.
Accurate microbiological diagnosis comprises molecular detection or quantitative buffy coat
analyses, each of which enhances sensitivity.
Genomic DNA was extracted from patients' buffy coat
samples according to the blood and body fluid protocol of the QIAamp DSP DNA Blood Mini Kit.
Blood specimens were collected from all 117 deer, and buffy coat
fractions were stored at -80[degrees]C for further analysis.
It delivers consistent results from a variety of clinical samples, including FFPE, blood, buffy coat
, swabs, and more.
For buffy coat
isolation and freezing at BioCytics, whole blood was collected in BD Vacutainer cell preparation tubes (BD BioSciences).
Results of quantitative buffy coat
analysis were positive for malaria trophozoites, schizonts, and gametocytes.
If there is a thick buffy coat
or a large urine-sediment button, these procedures may be necessary to allow for better distribution and interpretation of the sample.