A borate buffer solution
(0.1 mol L-1) was used to prepare a 1.0 x 10-5 mol L-1 BSA solution, ODEX solutions with different concentrations, and mixed solutions of ODEX-BSA (1.0 x 10-5 mol L-1) at different concentrations.
For the purpose of the peak current 4x[10.sup.-5] M TX was examined with DPV for 1 M acetate buffer solution
at pH 5.5 on the same MWCNT-modified GCE stored at room temperature 2 months.
(Replacement kits were issued to participants upon request.) Using [H.sub.2]O dilution buffer solution
or [D.sub.2]O exchange buffer solution
, as appropriate, laboratories promptly diluted the Fab stock solutions to the concentration suitable for use in HDX-MS studies.
The enzymeen-trapped copolymer was electrochemically formed using a mixture of 0.375 M of Py and 0.125 M of EDOT monomers in 0.05 M of NaDBSA, 1 mL 0.1 M of phosphate buffer solution
(at pH 6.7) containing 2.94 mg/mL GaOx (40 units), and 2.0 mg [beta]-galactosidase (20 units).
2 shows the cyclic voltammograms for 20 [micro]mol [L.sup.-1] AML and 50 [micro]mol [L.sup.-1] ATOR in BR buffer solution
(pH 4.0) on the anodically pretreated BDD electrode at the scan rate of 40 mV s-1.
1, recTnT incubated in a buffer solution
; 2, recTnT incubated ina buffersolution with thrombin (3 NIH units/mL); 3, recTnT incubated in a buffer solution
with hirudin-pretreated thrombin; 4, ITC ternary complex incubated in a buffer solution
; 5, ITC ternary complex incubated in a buffer solution
with thrombin (3 NIH units/mL); 6, ITC ternary complex incubated in a buffer solution
with hirudin-pretreated thrombin; 7, cTnT from AMI serum sample; 8, recTnT incubated in NHS; 9, recTnT incubated in NHS pretreated with heparin (300 USP units/ mL); 10, recTnT incubated in NHS pretreated with hirudin (10 IU/mL); 11, recTnT incubated in heparin NHP.
The enzyme assay was carried out with the substrate prepared in buffer solutions
of varying pH: 20 mmol/L PBS for pH 6.0 and 7.0; 20 mmol/L N[H.sub.4]HC[O.sub.3] buffer solution
for pH 8.0; 20 mmol/L boric acid-borax buffer solution
for pH 8.4 and 9.0; and 20 mmol/L borax-NaOH buffer solution
for pH 9.6.
Table 1: The chemical ingredients of McDougall buffer solution
(2005) stated that the use of buffer solution
had little influence on gas production and only minimized pH variations.
The biodegradation of polymeric material Sh-AA-MBA was carried out by following protocol: The polymeric material was immersed in phosphate buffer solution
of pH 7.4 along with enzyme lipase (1 mg [mL.sup.-1]).
Control Group (n = 5): 5 ml buffer solution
with lipid fraction + 2 ml neutral buffer + 1 ml DMSO
Always use fresh buffer solution
for calibration, this ensures the buffer being used is not contaminated.
A sodium phosphate saline buffer solution
at 6.0 pH was chosen as the eluent.
In this research, various concentrations of multi-walled carbon nanotubes were prepared in phosphate buffer solution
, and were injected into each mouse in two steps.