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Related to brucellae: Brucella melitensis
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an aerobic Gram-negative coccobacillus that causes brucellosis

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In the context of a clinically compatible illness, brucellosis is confirmed by a fourfold or greater rise in Brucella agglutination titer between acute-and convalescent-phase serum specimens obtained at least 2 weeks apart, isolation of Brucellae in culture, or demonstration of organism presence by specific immunohistochemical staining (3).
Brucellae are found in a great number in the milk and abortive products of infected animals and thus brucellosis has become an occupational disease for farmers, veterinarians, slaughterhouse workers and lab technicians [6].
Development of brucellae as a weapon was halted in 1967, and then-President Nixon later banned development of all biological weapons on November 25, 1969.
Antibody reactivity to Omp31 from Brucella melitensis in human and animal infections by smooth and rough Brucellae.
abortus species are made up of overlapping epitopes and the practical implication is that the single antigen can be used for the diagnosis of brucellosis caused by smooth strains of Brucella (30) Brucellae sLPS is the main virulence factor which evinces strong immune responses and widely used for preparation of various diagnostic assays (30).
Brucellae have been found in wildlife, such as bison, elk, and wild boar, potentially posing a threat for zoonosis (2).
Brucellae are class 3 organisms and potential agents of bioterrorism.
Therefore, serologic tests that use suspensions of smooth brucellae are not useful in diagnosing B.