The reaction then stopped by the addition of Tris-HCl buffer (125 mM), pH 6.8 containing 10 of glycerol, 5 of [beta]-mercaptoethanol, 2 of SDS and 0.001% of bromophenol blue
. Samples were boiled for 5 min.
Note on the use of bromophenol blue
for the histochemical recognition of protein.
Jugade, "Spectrophotometric determination of cefixime trihydrate in pharmaceutical formulations based on ion-pair reaction with bromophenol blue
," Analytical Chemistry Insights, vol.
In the previous study, we used sulphonphthalein acid including bromophenol blue
, bromocresol green, and bromothymol blue to determine ciprofloxacin pharmaceutical formulations and achieved good results .
Samples were diluted with 4X Laemmli buffer, consisting of 250mM Tris, pH6.8, 8% SDS (w/v), 8% dithiothreitol (DTT) (w/v), 40% glycerol (v/v), and 0.0008% bromophenol blue
(w/v), boiled for 5 minutes, and resolved by Tris-glycine SDS-PAGE, through 1 mm thick 15% T and 3% C running gel.
SSCP analysis: A volume of 2 uL PCR product was transferred in an Eppendorf tube, mixed with 7 uL gel loading solution containing 98% formamide, 0.025% bromophenol blue
, 0.025% xylene cyanol, 20 mmol/L EDTA (pH 8.0) and 10% glycerol.
For each [[theta].sub.t] tested, three hydrodynamic assays of stimulus-response were carried out in pulse type using eosin Y and bromophenol blue
as tracers, with injection volume of 10 mL and injection time of 10 s.
Five young egg-laying females of each nematode population were dissected from roots, macerated in 20 ml of extraction buffer (20% sucrose, 2% Triton X-100 and 0.01% Bromophenol blue
) and squashed to release body contents.
The samples were loaded with Bromophenol blue
loading dye, taking care not to puncture the well bottoms.
Abdel-Rehim, High-Dose Film Dosimeters Based on Bromophenol Blue
or Xylenol Orange Dyed Polyvinyl Alcohol.
40 uL extract of each sample was mixed with 20 uL sucrose and 10 uL bromophenol blue
, then 50 uL from each mixture was applied to each well.
The histochemical stains used were: Xylidine Ponceau (Melo and Vidal, 1980) and Bromophenol Blue
(Pearse, 1985) for the detection of proteins; Periodic acid-Schiff (PAS) and simultaneous PAS and Alcian Blue (Junqueira and Junqueira, 1983) for polysaccharides; vonKossa method (Junqueira and Junqueira, 1983) for calcium detection; and Nile Blue (Lison, 1960) and Sudan Black B (Junqueira and Junqueira, 1983) for lipids detection.
employed phloxine B and bromophenol blue
as the sensitizers of dye-sensitized solar cells, and the devices were characterized using UV-Vis spectra, FT-IR spectra, fluorescence spectra, and current-voltage characteristics.
Two microliters of bromophenol blue
dye was loaded with all reaction mixer samples.
A 5 [micro]l aliquot of each PCR product was mixed with 5 [micro]l of loading dye (98% formamide, 10 mM EDTA, 0.025% bromophenol blue
, 0.025% xylene cyanol).