In the assays with bromophenol blue
, the long tailing phenomenon is less evident because is released faster than eosin Y, which probably remains adsorbed in the support medium, being discharged slowly in the effluent of the reactor.
is a tetrabromophenol sulfonaphthalein, widely used as a 'tracking dye' by the scientific community.
The cytochemistry staining and measurement of protein with mercuric bromophenol blue
7% agarose gel stained with ethidium bromide (10mg/mL) by loading 12 [micro]l of DNA preparation (2[micro]l extracted DNA, 2[micro]l gel loading buffer containing 25% bromophenol blue
and 30% glycerol, 8[micro]l sterilized deionized water).
Studies of complex formation between the bromophenol blue
and some important aminoquinoline antimalarials.
Ethanol precipitates were resuspended in isoelectro-focusing medium containing 8 mol/L urea, 20 g/L CHAPS, 20 mL/L Triton X-100, 8 g/L Pharmalyte 3-10 (Amersham Biosciences), 100 mmol/L dithiothreitol, 2 mL/L Tergitol NP7 (Sigma), and traces of bromophenol blue
Sections (5 [micro]m) were cut using a Microm HM 340 microtome and stained with Hematoxylin and Eosin for structural examination or with Mercuric Bromophenol Blue
to elucidate the location of proteins (Chapman 1975).
The list of protocols to measure carnitine started in 1953, with a bioassay published by Fraenkel, followed by a method using bromophenol blue
(43), and from then several improvements have been made.
A total of 7 [micro]L of PCR products with 3 [micro]L of loading buffer containing the tracking dye bromophenol blue
were mixed and loaded onto the gel.
The gels ran until the bromophenol blue
dye passed out from the bottom of the gel.
The same amount of protein samples was loaded on the gel and separated at 150 V until the bromophenol blue
reached the bottom of the gel.
Then after adding the loading dye Bromophenol blue
the samples were loaded in the gel and were run at a constant voltage of 220V and current of 60mA.
Second dimension SDS -PAGE was first run at a current of 15 inA/gel for 1 hr and then at a constant current of 30 mA/gel at 10[degrees]C until bromophenol blue
reached the gel [bottom.
s] , 20 mg of total sperm protein and medium (sperm cultured medium) were mixed with 2 [micro]l/10 [micro]l FOZ loading buffer (5% bromophenol blue
, 10% SDS, 2% glycerol) and reacted on ice for 5 min.
Eight micrometer sections were made using a Reichert-Jung Leica model 2040 Autocut, and the following techniques were used: Toluidine Blue (routine), Periodic Acid of Schiff (PAS, carbohydrates), Bromophenol Blue
(proteins) (Pearse, 1985) and Nile Blue (lipids) (Hawes and Satiat-Jeunemaitre, 2001).