Brain heart infusion agar
promotes the growth of organism which requires high protein from calf brain and beef heart infusion and amino acid source for energy and growth.
The mold was then subcultured to Brain Heart Infusion agar
and incubated at 37[degrees] C, which yielded yeast colonies.
Anaerobic culture on brain heart infusion agar
isolated actinomyces species within 72 hours (Figure- 4) Chest X ray- Normal, X ray Right foot-Osteomyelitis of 4th, 5th metatarsal bone (Figure 5), KOH Preparation- Negative for fungal elements, Biopsy-Excision biopsy was done (four whitish yellow granules were obtained while doing deep exsion biopsy), Histopathology-Epidermis shows pseudo epitheliomatous hyperplasia.
The inoculum from BHI broth was streaked onto the Brain Heart Infusion Agar
, MacConkey's Agar, Blood Agar and Eosin Methylene Blue Agar plates in order to assess different growth and culture characteristics of all the pathogens present in the milk samples.
Marine Agar and Brain Heart Infusion Agar
with 1% NaCl were both chosen for plating bacteria because of their nonselective properties.
The yeast form was achieved by incubating the isolate on brain heart infusion agar
on slants at 35[degrees]C [+ or -] 2[degrees]C for 7 days in a single subculture.
(2,5,6) For the detection of resistance to vancomycin, the methods used included; a) vancomycin screen agar test using 6 [micro]g/ml of vancomycin incorporated in Brain Heart infusion agar
, and b) MIC of vancomycin (VHB Life Sciences) by macrobroth dilution method with dilutions ranging from 0.5 [micro]g/ml-128 [micro]g/ml.
The strains were maintained in heart infusion agar slants (HIA, Difco, USA), and prior to assay, the cells were grown overnight at 37[degrees]C in brain heart infusion agar
Each colony was used to inoculate a brain heart infusion agar
slant and a tube of brain heart infusion broth.
This isolate was tested for antimicrobial susceptibility by disk diffusion; a vancomycin-agar screen plate (brain heart infusion agar
containing 6 [micro]g/mL vancomycin) also was inoculated.