Differences in human immunodeficiency virus type 1 (HIV-1) anti-p24 reactivities in serum of HIV-1-infected and uninfected subjects: analysis of indeterminate Western blot
* Pretreating the blot
in preparation for hybridization with a labeled DNA probe (the fragment of DNA that will seek out and hybridize to its complementary target in the patient DNA)
were hybridized with [sup.32]P-labeled mitochondrial (ND1) and nuclear (18S rRNA) probes, and the radioactive signals were quantified with a PhosphorImager (Fig.
To determine if the gene expression profiles on the array could be verified by other techniques that monitor mRNA expression, we compared the expression profiles of several genes on the arrays (Vtg 2, choriogenin 2, and transferrin) to their profile by Northern blots
and DD RT-PCR.
Diagnostic screening using ASOs for clinically relevant base substitutions (mutations/polymorphisms) frequently uses a dot blot
Protein was detected with a Phototope-HRP Western blot
detection kit (Cell Signaling Technology).
included serial dilutions of a single preparation of adult midbrain, which was then used to construct a standard curve to normalize the values across blots
Autoradiographic images from the Western blots
were digitally scanned and densitometric measurements for phospho-ERK and total ERK signals were performed using the NIH Image program.
"On December 28, NCB received information that three LSD blots
will be shipped through DTDC courier to Jaipur for the upcoming New Year celebrations.
Kerner, who described his inky creations as "chance inkblots," seems to have stressed their arcane provenance, even suggesting that "the forms assumed by the blots
were sometimes of supernatural origin--'figures from Hades'" (Pichot 1984, 594-95).
The article was found to contain duplicated images in Figure 4(a), in which the IL6 blots
are identical to the p-ERK5 blots
READER Willie Quinn claims wind farms are "expensive, inefficient, unworkable blots
on the landscape".
When comparing relative protein expression levels in western blots
, normalization methods are commonly used to control for technical errors and inconsistencies that arise during sample preparation, loading and transfer steps.
The software automates total protein normalization of multiplex fluorescent western blots
by extracting image data directly from the ChemiDoc MP imager.