(52.) See Akulapalli, supra note 48 (reiterating lymph and blastema
theories of cancer).
Mescher, "Transferrin and the trophic effect of neural tissue on amphibian limb regeneration blastemas," Developmental Biology, vol.
Tassava, "Cloning and neuronal expression of a type III newt neuregulin and rescue of denervated, nerve-dependent newt limb blastemas by rhGGF2," Journal of Neurobiology, vol.
Blastema cells then accumulate underneath the healed epidermis, which forms a thickened structure at its apex, called the apical epithelial cap (AEC) [21, 22].
The pattern of mitosis in anterior and posterior regeneration in Dugesia (G) tigrina, and a new proposal for blastema
With a technique called subtractive hybridization, the researchers collected fragments of genes turned on in blastemas when a planarian regenerates its tail, its head, or both and compared them to genes normally active in the head and tail.
The investigators detected dozens of DNA fragments specific to regenerating blastemas, some belonging to genes that become active in the initial hours of the process.
The resulting blastema, a mass of unspecialized cells, proliferates rapidly to form a limb bud.
Examination of dissected animals and crab exuviae revealed that this mode of regeneration is produced by continuously generating blastemas at the proximal ends of the previous regenerates.
(1996) transplanted the hindlimb distal blastema to the forelimb proximal stumps of newt limbs.
It is known that limb regeneration does not take place by direct outgrowth but by the production of undifferentiated blastema cells.
Following the epithelial healing and formation of the wound epidermis, a mass of proliferating mesenchymal cells, termed the blastema, is formed in a region adjacent to the wound epidermis.
In fish regeneration, it has also been believed that, as in the urodele limb regeneration, the blastema is formed by cell dedifferentiation.
Planarian regeneration involves the generation of new tissue at the wound site via cell proliferation (blastema formation), and the remodeling of pre-existing tissues to restore symmetry and proportion (morphallaxis).
This process commences with the formation of blastema, which is composed by neoblast which are undifferentiated and totipotent cells.