Genotyping on Licor 4300 DNA Analyzer: The amplified PCR products of M-13 tailed markers were resolved on PAGE gel prepared and fixed for Licor 4300 DNA Analyzer which revealed autoradiograms
image was digitized, unwarped, and straightened, by using the DENDRON software database (25).
A representative AFLP autoradiogram
was developed (Fig.
Digital images of the edited autoradiograms
indicating operator determination of band placement were transmitted electronically to the CDC network coordinator for comparison.
For one pair of great reed warblers, we had nine young on the same autoradiogram
because they raised two clutches in the same season.
The patterns of the existing pool of proteins ([ILLUSTRATION FOR FIGURE 6A OMITTED]: Coomassie-stained proteins) were different than those of the newly synthesized proteins ([ILLUSTRATION FOR FIGURE 6B OMITTED]: autoradiogram
of labeled proteins), with no detectable synthesis of the two high-molecular-weight protein classes at any larval stage (note lack of signal in [ILLUSTRATION FOR FIGURE 6B OMITTED] autoradiogram
Each isoform on the blot was visualized with a horseradish peroxidase-conjugated goat anti-mouse antibody, and the signal was detected on an autoradiogram
by chemiluminescence as described above.
Figure 1 shows a typical autoradiogram
using the single primer pair AAC/CTT combination on a series of 30 opium poppy samples.
Positive samples containing HSV show a characteristic "retarded" band on the autoradiogram
of the electrophoresis gel.
showed that traces of 14C-dansyl-GP and 14C-dansyl-G were present in the medium simultaneously with the hexapeptide ([ILLUSTRATION FOR FIGURE 6 OMITTED], lane A).
d) Measurement of the mean TRF length by quantitative densitometry of the smear pattern of an autoradiogram
may not be accurate.
Figure 2 represents the autoradiogram
of the Southern analysis conducted on the transgenic soybean plants hybridized with the GUS-specific probe (the banding pattern with the CP4-specific probe was identical, data not shown).
of allele-specific gene expression studies by restriction isotyping in our five patients is given in Fig.
from experiment 4 [ILLUSTRATION FOR FIGURE 2A OMITTED], in which specimens of Aiptasia pallida were incubated in a temperature series from 22 [degrees] to 35 [degrees] C (Table I), was analyzed with densitometry to compare quantitatively the densities of the hsp68 and hsp72 bands from animals exposed to the various temperatures.
This method, currently based on the use of membrane as solid support [24,25] and autoradiogram
analysis, is tedious and seems difficult to be done by laboratory technicians unskilled in molecular biology.