image was digitized, unwarped, and straightened, by using the DENDRON software database (25).
Digital images of the edited autoradiograms
indicating operator determination of band placement were transmitted electronically to the CDC network coordinator for comparison.
Signal was detected on autoradiograms
by chemiluminescence per the manufacturer's instructions (Amersham Life Science).
TRF length estimated by Southern blotting does, however, have disadvantages: (a) TRF does not indicate pure telomere repeat length but includes various unknown lengths of subtelomic sequences; (b) to estimate TRF length, genomic DNA should be purified as intact (unsheared) as possible; (c) the mean TRF length estimated by reading smear patterns of autoradiograms
may be inaccurate; and (d) Southern blotting is time consuming.
were analyzed by quantitative scanning densitometry using a Model GS-700 Imaging densitometer and the Molecular Analyst software (Bio-Rad).
were obtained after 30 days (-4[degrees]C) exposure in light-tight cassettes to Kodak Professional D-19 (Sigma) film and developed in Kodak Dektol (Sigma Chemical Co.
The processed blots were exposed to X-ray film, and the autoradiograms
were analyzed using a scanner and the GelQuantNET software (http://biochemlabsolutions.
Other admirable qualifies include the wherewithal to covalently bind nucleic acid samples to membranes in less than 30 seconds (240 times faster than vacuum-oven baking, according to the firm), and enhance sample visibility on autoradiograms
by up to 445 percent.
Those include references to must-read literature; genetic and physical maps of chromosomes, indicating the approximate location of disease-resistance genes of interest; and autoradiograms
that show the distinctive bands associated with such genes.
1996) and the autoradiograms
read by two different people to confirm the sequence.
2]R-expressing neurons, as displayed by the low binding densities in some midbrain regions of the representative autoradiograms
As the FBI's leading DNA expert later conceded, there were significant problems when DNA evidence was first introduced in court: "The initial outcry over DNA typing standards concerned laboratory problems: poorly defined rules for declaring a match; experiments without controls; contaminated probes and samples; and sloppy interpretation of autoradiograms
Loss of heterozygosity was scored by visual comparison of the intensity of alleles on autoradiograms
They are also used to read chromatograms, protein gels, and autoradiograms
Perform molecular techniques adequately Refrigerated microcentrifugation and storage Develop autoradiograms
and photograph agarose gels Produce high quality solutions Sterilize solutions/instruments