1,3-Diaminopropane (with 2 amino-nitrogens) and malonamide (with 2 amide-nitrogens) serve as approximate models for the potential 6-atom ring complex formed by asparagine
(1 amino-nitrogen and 1 amide-nitrogen).
PATENTS RELATED TO ASPARAGINE
PRODUCTS BY YEAR, 1998-2008
Prior to Erwinaze's approval there were two asparagine
specific enzyme products - Elspar (asparaginase injection) and Oncaspar (pegaspargase) - approved by FDA to treat patients with ALL.
The 23-compound calibration mixture (Std AA) used for this experiment was prepared by adding glutamine, asparagine
, taurine, citrulline, and [gamma]-aminobutyric acid (GABA) to a commercial 18-amino acid calibration mixture (Sigma) and diluted with the dabsylation buffer to achieve a final concentration of 1 nmol per 20 [micro]L for each of the amino acids.
The enzyme destroys the plasmatic amino acid asparagine
, which leukemia cells require to continue their rapid, malignant growth.
Simplot's first application of Innate(TM) Technology involves improved potato varieties, which differ from their conventional counterparts in three ways: 1) reduced black-spot bruising; 2) reduced degradation of starch to sugars during storage; and 3) reduced asparagine
All resistant isolates contained a point mutation resulting in a serine to asparagine
change at amino acid 31 (S3IN) of the M2 protein.
A third recombinant insulin that is longer acting is insulin glargine (Lantus[R]; Aventis Pharmaceuticals), which differs from regular human insulin by the substitution of glycine for asparagine
at position A21 and by the addition of two arginine residues to the COOH terminus of the B chain.
L-asparaginase is an enzyme that depletes the amino acid asparagine
, which certain leukemic cells are dependent upon for survival.
About Oncaspar (pegaspargase): While normal cells can produce asparagine
, leukemic cells are unable to produce enough asparagine
to survive on their own.
A single nucleotide difference at position 976 was responsible for the occurrence of an uncharged asparagine
(codon AAC) in MON-2 or a negatively charged aspartic acid (codon GAC) in MON-1, MON-18, and MON-37 sequences.
The primary proteolytic activity of ASNase is the conversion of the amino acid asparagine
to aspartic acid and ammonia.
This study evaluated the utility of Oncaspar in solid tumors and lymphomas as well as assessed the correlation of Oncaspar activity with cellular levels of asparagine
Administering L-asparaginase results in the depletion of asparagine
circulating in the blood, which starves the leukemic cells and results in their death.
Subsequent electrophoresis demonstrated an abnormal hemoglobin band, which was identified by DNA sequencing to have a base exchange leading to substitution of asparagine
for lysine at residue 66 in the [beta]chain (8).