Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion.
AB= Aniline blue staining, TB= Toluidine blue staining.
The B & K and B & K + aniline blue methods gave 40% and 34% and 36% and 40% declines in pollen viability, respectively, for DP 5415RR and SG 125RR.
In order for a boll to have yielded 30 or more seeds (the lowest mean seed set of bolls from nonglyphosate treated plants), pollen viability must have exceeded 82, 95, 76, and 84% as measured by the B & K, Alexander's stain, B & K + aniline blue, and FCR methods, respectively (Fig.
The addition of aniline blue to B & K media appeared to inhibit pollen germination and tube growth.
More than 95% of pollen grains from both morphs of both species stained darkly with aniline blue
dye, indicating high pollen viability.
Sequential staining of callose by aniline blue
and lacmoid for fluorescence and regular microscopy on a durable preparation of same specimen.
In the present study, four different cytohemical staining methods including Toluidine Blue, Aniline Blue, Chromomycin A3 and Acridine Orange assays were applied in order to assess different aspects of sperm DNA integrity (31).
Our results showed that when the cases were divided in two groups according to chromatin abnormality, fertilization rates were significantly increased in the group with less than 10% abnormal chromatin threshold using Aniline Blue. Such finding may indicate that protamine deficiency has considerable affect on fertilization rate.
The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme.
Aniline blue is soluble in water but not in methyl salicylate or BBDP, whose refractive indices (1.522 and approximately 1.542, respectively, Weast, 1985) are ideal for clearing and observing whole grass pistils (Young et al., 1979; Crane and Carman, 1987).
rectisetus (1,9020-1) 33 3 Data obtained from pistils of the second and third collections, which were cleared in the sucrose-based aniline blue solution A (Table 1), analyzed for callose fluorescence, recleared in BBDP, and re-analyzed for details of meiocyte development by means of Nomarski optics, are presented in Tables 3 and 4.
Pistils were cleared in aniline blue clearing medium, scored for callose fluorescence using fluorescence microscopy, recleared in BBDP, and staged using interference contrast microscopy.
If the same is true of other diplosporous species, then aniline blue clearing media should prove useful for identifying new diplosporous species and for determining the frequency of facultative sexual expression (Peel et al., 1997).
An overall decrease in MMC callose fluorescence was observed when pistils were cleared in BBDP first and then recleared in aqueous aniline blue. Thus, studies aimed at quantifying callose deposits and correlating them to developmental events, such as aposporous initial development, should involve rapidly killed and fixed tissues that are analyzed immediately.