Aerobic culture of aspirate was negative; anaerobic culture
grew Actinomyces meyeri that was susceptible to penicillin and clindamycin.
Technical limitations of anaerobic culture
arise mostly due to toxic oxygen exposure making isolation of anaerobes from clinical specimen quite difficult.
predominately grow in anaerobic culture
bottles, and clostridial sepsis usually affects elderly persons with abdominal conditions, malignancy, or immune suppression (11,12).
A review of our culture records indicated that virtually no significant microorganisms were isolated only from the anaerobic broth used to back up the anaerobic culture
The best specimen for anaerobic culture
is obtained by using a needle and syringe.
amnionii was previously recovered in anaerobic culture
of the amniotic fluid of a woman after intrauterine fetal demise (2).
Typical examples of these are throat cultures for organisms other than beta hemolytic streptococci; identification of more than three pathogens in a specimen; cultures of oropharyngeal material, bowel contents, perirectal abscesses, decubiti, and Foley catheter tips, and anaerobic culture
of surface wounds; and antibiotic susceptibility determinations on obvious contaminants, commensal species from sites they normally inhabit, or species with predictable susceptibilities.
on brain heart infusion agar isolated actinomyces species within 72 hours (Figure- 4) Chest X ray- Normal, X ray Right foot-Osteomyelitis of 4th, 5th metatarsal bone (Figure 5), KOH Preparation- Negative for fungal elements, Biopsy-Excision biopsy was done (four whitish yellow granules were obtained while doing deep exsion biopsy), Histopathology-Epidermis shows pseudo epitheliomatous hyperplasia.
directly from specimen yielded similar spore-forming, gram-positive bacilli.
Then, the glass tubes were sent to the clinical microbiology laboratory, and a few drops were taken from glass tubes and applied on the plates in order to develop aerobic and anaerobic cultures
. Therefore, the nasal discharge was usually in contact with the solution for more than 30 minutes before inoculation.
Both 72-hour aerobic cultures and anaerobic cultures
held for six days were negative.
Both aerobic and anaerobic cultures
were maintained at a temperature of 35[degrees]C (+/-2[degrees]C) during the incubation period.
In view of septic thrombosis, anaerobic cultures
were advised but were found to be negative.
Aerobic and anaerobic cultures
were taken, with negative results.