In this review, we focused on the detection of FGFR1 amplification by FISH.
The variability of FGFR1 amplification rates as determined by FISH is related to differences in the definition of a positive result and in interpretation of results.
Fibroblast Growth Factor Receptor 1 Amplification in Nonsmall Cell Lung Cancer Epidemiology
The best calculation of the amplification factor is when we have data from borehole seismographs.
It was observed (Nakamura, 1989) that the vertical component cannot be amplified around the frequency where horizontal component receives large amplification ([A.
Dominating peak have information about amplification and the frequency is related to principal frequency of the cover.
The use of the Ribo-SPIA technology for global amplification of mRNA in biological samples and subsequent quantification of defined sets of transcripts of interest has been validated in a mouse model for study of systemic HIV-1 infection (4).
Quantification of DNA targets and amplification of products, as well as cDNA (before and after amplification), were carried out with an ABI PRISM[TM] 7700 sequence detector (Applied Biosystems) or an MJ Opticon (MJ Research).
MYCC amplification was analyzed using MYCC probe located at region 8q24-q26 and compared against a centromeric probe for chromosome 9 (D9Z1).
The number of signals for the chromosome 9 centromere compared against the number of MYC signals determined the presence of amplification.
PCR was performed by using AmpFLSTR[R] Profiler Plus[TM] PCR amplification kit (Perkin-Elmer Applied Biosystems, Foster City, California) and GenePrint[TM] Sex Identification System-Amelogenin (Promega Corporation, Madison, Wisconsin).
Each reaction for amplification using the AmpFLSTR[R] Profiler Plus[TM] kit contained 20.
Used in conjunction with expression microarrays, GenoSensor genomic microarrays can help researchers determine not only if a gene is present, but also whether the gene is found in abnormally high numbers, and if abnormal gene amplification
is correlated with overexpression of an important protein.
The second element is the time and labor associated with the past amplification analysis of the product.
End point" detection or real time monitoring of the amplification reaction may be performed using commercially available systems.