Therefore any condition that would completely inactivate a virus while leaving its nucleic acid amplifiable
would result in the models overestimating AGI incidence.
Regarding the tested protocols, the Nucleospin technique was the only one able to produce amplifiable
DNA from refined vegetable oils.
A simple and efficient method for PCR amplifiable
DNA extraction from ancient bones.
Risca, "Genomic Steganography: Amplifiable
Microdots", Cambridge, 2005.
Though Southern blot analysis was for long the method of choice to establish the exact breakpoints (23), since this method was time-consuming and cumbersome, the two recent methods, i.e., Multiplex Amplifiable
Probe Hybridization (MAPH)24 and MLPA18-20, has greatly simplified this analysis, where MLPA has become the method of choice.
The very large amplifiable
element AUD2 from Streptomyces lividans 66 has insertion sequence-like repeat at its ends.
We found no strong correlation between the number of amplifiable
species (1-8) and either (i) the cloned allele size (109-299 bp; r = -0.012, P = 0.934) or (ii) the length of the repeat motif (2-4 bp; r = 0.079, P = 0.602).
Isolated DNA was completely digestible with restriction enzymes (EcoR1 and Mse 1) and amplifiable
in the polymerase chain reaction.
Neither primer sets produced a PCR product, indicating that DNA degradation had occurred to the extent that amplifiable
DNA could not be isolated from these four specimens.
DNA is available in two minutes or less.
Extraction of amplifiable
DNA is a frequent problem when working with degraded specimens like bone samples.
In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable
markers, and selecting for increased copies of the one or more amplifiable
markers located on the integrated vector.
Nuclear microsatellite DNA from the A7 and A24 loci, and mtDNA from a 485 bp segment of the cytochrome b gene were all amplifiable
, to varying degrees, depending upon the laboratory or field conditions to which they had been exposed.
If all the primers are considered simultaneously, the average number of bands per plant (data not shown), and the total number of bands amplifiable
(Table 2, numbers in brackets) are lowest for the inbred female line b220.127.116.11, and highest for the three cultivars, Fibranova, Fibrimon, and Northern Lights.
1989; Parejko and Dodson 1991; Spitze 1992; Tollrian 1995; Spitze and Sadler 1996) are amplifiable
characters with a continuous trait distribution once a threshold of inducer has been exceeded.