Therefore any condition that would completely inactivate a virus while leaving its nucleic acid
amplifiable would result in the models overestimating AGI incidence.
Regarding the tested protocols, the Nucleospin technique was the only one able to produce
amplifiable DNA from refined vegetable oils.
A simple and efficient method for PCR
amplifiable DNA extraction from ancient bones.
Risca, "Genomic Steganography:
Amplifiable Microdots", Cambridge, 2005.
Though Southern blot analysis was for long the method of choice to establish the exact breakpoints (23), since this method was time-consuming and cumbersome, the two recent methods, i.e., Multiplex
Amplifiable Probe Hybridization (MAPH)24 and MLPA18-20, has greatly simplified this analysis, where MLPA has become the method of choice.
The very large
amplifiable element AUD2 from Streptomyces lividans 66 has insertion sequence-like repeat at its ends.
We found no strong correlation between the number of
amplifiable species (1-8) and either (i) the cloned allele size (109-299 bp; r = -0.012, P = 0.934) or (ii) the length of the repeat motif (2-4 bp; r = 0.079, P = 0.602).
Isolated DNA was completely digestible with restriction enzymes (EcoR1 and Mse 1) and
amplifiable in the polymerase chain reaction.
Neither primer sets produced a PCR product, indicating that DNA degradation had occurred to the extent that
amplifiable DNA could not be isolated from these four specimens.
Amplifiable DNA is available in two minutes or less.
Extraction of
amplifiable DNA is a frequent problem when working with degraded specimens like bone samples.
In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more
amplifiable markers, and selecting for increased copies of the one or more
amplifiable markers located on the integrated vector.
Nuclear microsatellite DNA from the A7 and A24 loci, and mtDNA from a 485 bp segment of the cytochrome b gene were all
amplifiable, to varying degrees, depending upon the laboratory or field conditions to which they had been exposed.
If all the primers are considered simultaneously, the average number of bands per plant (data not shown), and the total number of bands
amplifiable (Table 2, numbers in brackets) are lowest for the inbred female line b92.73.2.13, and highest for the three cultivars, Fibranova, Fibrimon, and Northern Lights.
1989; Parejko and Dodson 1991; Spitze 1992; Tollrian 1995; Spitze and Sadler 1996) are
amplifiable characters with a continuous trait distribution once a threshold of inducer has been exceeded.