Therefore any condition that would completely inactivate a virus while leaving its nucleic acid amplifiable
would result in the models overestimating AGI incidence.
Considering the few successful reports and difficulties involved in obtaining amplifiable
DNA from refined vegetable oils, this research can be considered a step toward being able to trace refined oils in order to clarify authenticity issues and concerns about the use of genetically modified organisms.
Comprehensive detection of genomic duplications and deletions in the DMD gene, by use of multiplex amplifiable
Isolated DNA was completely digestible with restriction enzymes (EcoR1 and Mse 1) and amplifiable
in the polymerase chain reaction.
Neither primer sets produced a PCR product, indicating that DNA degradation had occurred to the extent that amplifiable
DNA could not be isolated from these four specimens.
quality of DNA samples was assessed by [beta]-globin reference gene polymerase chain reaction (PCR) of the DNA extracted from the sample.
DNA is available in two minutes or less.
Extraction of amplifiable
DNA is a frequent problem when working with degraded specimens like bone samples.
In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable
markers, and selecting for increased copies of the one or more amplifiable
markers located on the integrated vector.
Nuclear microsatellite DNA from the A7 and A24 loci, and mtDNA from a 485 bp segment of the cytochrome b gene were all amplifiable
, to varying degrees, depending upon the laboratory or field conditions to which they had been exposed.
If all the primers are considered simultaneously, the average number of bands per plant (data not shown), and the total number of bands amplifiable
(Table 2, numbers in brackets) are lowest for the inbred female line b92.
1989; Parejko and Dodson 1991; Spitze 1992; Tollrian 1995; Spitze and Sadler 1996) are amplifiable
characters with a continuous trait distribution once a threshold of inducer has been exceeded.
It does so by exploiting the unique microfluidic properties of integrated fluidic circuits (IFCs) to detect only amplifiable
molecules within the sample mixture.
Nearly two-thirds of samples in this study were found to contain detectable amplifiable
host DNA, and p53 mutations were observed in a number of the NSCLC patient samples (although oddly, when compared to invasive biopsy specimens on the same cases, the specific mutations noted were discordant, suggesting heterogeneity in the tumor cells).
ISSR is a PCR based technique that amplifies DNA segments present between two identical microsatellites oriented in opposite direction and at relatively amplifiable
distance along the DNA strand (Reddy et al.