, and thymidine (HAT) were used to culture NG108-15 cells; however, aminopterin
in HAT was toxic to INS-1 cells (Figure 2(a)).
Hybridomas were grown in DMEM culture medium (Sigma, USA) containing 20% Fetal Calf Serum (FCS) (Seromed, Germany), penicillin (100 IU/ml) and streptomycin (100 [micro]g/ml) and supplemented with hypoxantine (1x[10.sup.-4] M), aminopterin
(4x[10.sup.-7] M) and thymidine (1.6x[10.sup.-5] M) (HAT) (Sigma, USA).
Methotrxate and aminopterin
are two uncommon antimetabolites that can induce cranial dysplasia and cleft palate in human.
Effect of aminopterin
in rheumatoid arthritis and psoriasis.
He had also developed another version of antifolate -- aminopterin
. Farber administered these two chemicals to his leukemia patients and the results were remarkable.
He had become aware of a report in the 1950s about the use of aminopterin
(a folic acid antagonist) to induce therapeutic abortion; this had resulted in a high incidence of neural tube defects and anencephaly in the aborted fetuses.
The resulting cells were distributed into micro-well plates in hypoxanthine aminopterin
thymidine medium which killed off the fusion partners, in that each well was left with one hybridoma cell.
Initially, the New York Agriculture Department released information that Aminopterin
, a form of rat poison, was found in some cat foods.
Less than a month later, on March 16, Menu Foods, an Ontario, Canada, manufacturer, announced a recall of 95 dog and cat food brands, all believed to contain aminopterin
, a rodent poison banned in the United States that can cause kidney failure in pets.
The fused hybridoma cells were propagated in RPMI 1640 supplemented with 100 mL/L fetal bovine serum, hypoxanthine, aminopterin
, and thymidine.