aminomethane


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Related to aminomethane: Trisamine, Tris buffer
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  • noun

Words related to aminomethane

a methyl with the hydrogen atom replaced by an amino radical

References in periodicals archive ?
After transference at 25 V: 100 mA for 1 h, the membrane was blocked with 5% non-fat dry milk diluted in 1X Tris (hydroxymethyl) aminomethane buffer (TBST), pH 8.4, for 2 h.
Zinc chloride (ZnCl2, purity=98.0%) and trihydroxy aminomethane (Tris, BR) were provided by Shantou Xilong Chemical Corporation of Guangdong and Sino-American Biotechnology Company of Shanghai, respectively.
The membrane was blocked using trihydroxymethyl aminomethane buffer salt + Tween-20 (TBST) containing 5% bovine serum albumin (Sigma) and washed with TBST (pH 7.4).
To ensure the stability of organelles and pH, 5 g mussel's tissue initially underwent a homogenization in chilled mortar by mixing at a rate of 1/10 w/v in Tris (hydroxymethyl) aminomethane 20 mM buffer, pH 7.8, and then was centrifuged at 10000 g for 30 min at 4[degrees]C.
Urease activity in soils was assayed by the buffer method (Tabatabai 1994) which involves the determination of the [NH.sub.4.sup.+] released when a soil sample is incubated with THAM (Tris hydroxymethyl aminomethane) buffer at the optimal pH (pH 9.0) with or without toluene, and urea (0.2M) at 37[degrees]C for 2h.
Urea, 2-mercaptoethanol, tris (hydroxymethyl) aminomethane (Tris), potassium dichromate ([K.sub.2][Cr.sub.2][O.sub.7]), sulfuric acid ([H.sub.2]S[O.sub.4]), sodium hydroxide (NaOH), and 1,5-diphenylcarbazide were purchased from Sigma-Aldrich.
After thepreliminary evaluations, sampleswere subjected to the initial dilution with prewarmed (37[degrees]C) Tris egg yolk citrate extender (TEYC) (tris-hydroxymethyl aminomethane 3.028% (w/v), sodium citrate 1.655% (w/v), fructose 1.250% (w/v), and egg yolk 20% (v/v); 100,000 IU penicillin G (sodium salt) and 100 mg dihydrostreptomycin were added in 100 mL of buffer).
The p-nitrophenyl concentration was determined colourimetrically using a UV/ VIS spectrophotometer (GBC) at 400nm, after extraction of supernatant with 1 mL Ca[Cl.sub.2] and 4mL NaOH (phosphatase activity) or 4 mL Tris (hydroxymethyl) aminomethane (THAM, pH 12) for [beta]-glucosidase and centrifugation at 3500rpm for 3 min.
2D SDS-PAGE, two dimensional sodium dodecyl sulfate poliacrilamide gel electrophoresis; CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio] propanesulfonic acid; Tris, tris(hydroxymethyl) aminomethane; DTT, Ditiotreitol; IEF, isoelectric focusing; EDTA, ethylenediaminetetraacetic acid; MALDI, Matrix-Assisted Laser Desorption/Ionization; NCBI, National Center for Biotechnology Information; IMT, Instituto de Medicina Tropical; PVDF, Polyvinylidene Fluoride
As compatibilizers, several polyalcohols, in some cases containing an amino group (for an easier incorporation in the epoxy matrix), such as polyethylene glycol 4000 (PEG), polysorbate 80 (TWEEN), and tris(hydroxymethyl) aminomethane (TRIS) were used, because they are expected to interact suitably with silicates and can be incorporated in the O-I matrix.
It was buffered at pH of 7.4 with 50 ml (tris-hydroxymethyl) aminomethane and 45 ml hydrochloric acid.
The thermokinetics of the reactions of nitroethane with ammonia at 15 oC and 25 oC and with trihydroxymethyl aminomethane at 15 oC and 30 oC had been studied with this method respectively.