macrophage

Alveolar macrophage cells (AMs) were maintained in DMEM/F12 containing 10% (v/v) fetal bovine serum at 37[degrees]C under a humidified atmosphere of 5% C[O.sub.2] and 95% air.

17, 2018 (HealthDay News) -- Electronic cigarette (e-cigarette) vapor condensate (ECVC) is significantly more toxic to alveolar macrophages than e-cigarette liquid (ECL), according to a study published online Aug.

Particle clearance may also occur from the alveoli, where deposited particles can be phagocytosed by alveolar macrophages and cleared by transport to the lung-draining lymph nodes [46, 47].

Our preliminary studies suggested that the chronic infections of the lower airway in COPD might involve dysfunction of the phagocytic ability of alveolar macrophages [2, 3, 10].

Reiches, "Alveolar macrophage subpopulations identified by differential centrifugation on a discontinuous albumin density gradient," American Review of Respiratory Disease, vol.

Ritzenthaler et al., "Chronic ethanol ingestion in rats decreases granulocyte-macrophage colony-stimulating factor receptor expression and downstream signaling in the alveolar macrophage," The Journal of Immunology, vol.

Mice exposed to 25ppm but not 250 ppb of arsenic had a modest increase in airway total leukocyte count that was driven by an increase in alveolar macrophages and lymphocytes (Figure 1d, e).

Key words: Alcohol consumption; alcohol use disorder; alcoholic lung; lung; lung disease; lung Injury; respiratory system; pulmonary system; alveolar macrophage; antioxidant

These all suggested that in contrast to apoptosis that had been observed in alveolar macrophages, necrosis was observed in alveolar epithelial cells [10, 14], which is an accidental cell death.

Measurements of cell viability weremade with Cell Titer 96Aqueous One Solution Cell Proliferation Assay [MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] with human alveolar macrophages as well as with human monocyte-derived macrophages (MDMs), incubated with different doses of 20nm and 110nmnAg for 24 hours.Thealveolar macrophages are primary human cells, cultured and exposed to nAg in serum free DCCM-1 media, with approximately 100,000 cells/well.

Laviolette, "Inhibition of human alveolar macrophage production of leukotriene B4 by acute in vitro and in vivo exposure to tobacco smoke," American Journal of Respiratory Cell and Molecular Biology, vol.

Extending the in vitro experiments using cell lines and isolated splenic macrophages, there has been an ex vivo study examining the effects of hyperglycemia on alveolar macrophage function [25].

Inflammation, caused by the release of mediators and oxygen radicals from alveolar macrophage and neutrophil activity in these regions, leads to fibrosis.

Remarkably, when the animals received dietary zinc supplements at doses comparable with those used to treat severe zinc deficiency in humans, the deleterious effects of chronic alcohol ingestion on GMCSF signaling and bacterial phagocytosis in the alveolar macrophage were reversed (Joshi et al.

Two cell lines, mouse lung epithelial (MLE12) cells and murine alveolar macrophage (MHS) cells, were employed in this study.