On the following ultrasound evaluations, between 25 and 60 days of pregnancy, the developmental pattern of the conceptus, including allantoic sac
development, yolk sac regression and umbilical cord formation, was similar to that described for mated or inseminated pregnant mares (GINTHER, 1992; 1998), jennies (MEIRA et al.
2 ml of stock viral suspension in each egg through the allantoic sac
Virus isolation testing was conducted by injecting pleural fluid, urine, and filtrates obtained from the ground tissues into the allantoic sac
of 10-day-old embryonated chicken eggs.
Excessive accumulation of allantoic fluid in allantoic sac
occurs as a single pathological factor in 85-90% of dropsical conditions affecting fetus and its membranes in bovines (Roberts, 1986 and Youngquist, 1997).
SARS coronavirus injected intratracheally into chickens, turkeys, geese, ducks, and quail, or into the allantoic sac
of their embryonating eggs, failed to cause disease or replicate.