In allantoic fluid
, lactic acid was only found after day 9 of incubation.
After estimation of HA titre separately the allantoic fluid
was pooled together considered as master seed virus and the HA titre and [EID.
The allantoic fluid
of embryonated SPF eggs infected with IBV samples were harvested and submitted to RNA extraction and thereafter cDNA synthesis.
Spot Agglutination Test: A drop of allantoic fluid
was placed on a glass slide and mixed with an equal volume of 5% freshly prepared washed chicken RBC's (Tiwari et al, 1996)
A correlation coefficient was employed to diagnose relationship between volume of allantoic fluid
and initiation of calving.
RNA extraction and cDNA synthesis: Viral RNA was extracted from 250 [micro]L of all the clinical specimens and from 140 [micro]L of the virus-containing allantoic fluid
(reference strains) using commercially available QIAamp Viral RNA Mini Kit (Qiagen GmbH, Hilden, Germany) as per manufacturer's instructions.
The allantoic fluid
was tested for the presence of haemagglutinins through haemagglutination test (HA) using 1% chicken erythrocytes.
Our earlier data had shown that infected allantoic fluid
retained virus without significant loss for 24 h at 37[degrees]C (Jadi and others, unpublished data).
The eggs are then cooled, before their tops are cut off and the allantoic fluid
, now containing the virus, is sucked out in a process called harvesting.
40) detected significant changes in amniotic and allantoic fluid
composition in clone and IVF pregnancies.
Utilization of FTA[R] Cards for the Molecular Diagnostic of Newcastle Disease Virus in Allantoic Fluid
Agglutinating virus in harvested allantoic fluid
was identified by spot agglutination assay using 10% chicken red blood cells (RBCs) and the identity of the virus was determined by NDV specific antisera through standard haemagglutination inhibition (HI) assay.
After chilling, haemagglutination test was performed on allantoic fluid
of each embryos to determine the presence of virus.
Hydrallantois is one of the gestational disorders with sudden increase in allantoic fluid
in allantoic cavity due to foetal membrane pathology leading to bilateral abdominal enlargement (Roberts, 1971).
3 showed embryonic mortality before 36 h in all inoculated eggs (4/4) and HA activity was detected (2 (8) and 2 (11)) in the allantoic fluid