alizarin red

The findings that demonstrated that the cells differentiated to osteogenic and adipogenic cells were illustrated with Alizarin red and Oil red staining respectively.

The osteogenic differentiation of the BMSCs in the osteogenic media after 21 days was positive and was confirmed by the presence of calcium deposits stained with Alizarin Red (figure 3).

The potential of osteogenic differentiation was evaluated after 21 days of incubation with the osteogenic stimulants (Stempro[R], Thermo Fisher) and stained with alizarin red at the end of the incubation period.

For each sample, microgrowth increments were measured from the shell margin to the Alizarin red staining point using microscopic image analysis software (Analysis Image Processing Version 5.

2% alizarin red is the most common method for determining viable and nonviable cells in areas where cells cannot be identified using specular microscopy (SPERLING, 1977; TAYLOR & HUNT, 1981).

A note on the staining of the skeleton of cleared specimens with alizarin red S.

Culture of BMSCs in osteogenic media lead to osteogenic differentiation of the BMSCs based on presence of calcium deposits after three weeks of staining with Alizarin red (Fig.

As for the diffuse band at 28 kDa, it is possible that the staining was non-specific; this band contains a large amount of protein and, since alizarin red can bind proteins to some extent, this staining may have reflected simply a large concentration of protein rather than its nucleation potential.

To confirm osteogenesis and adipogenesis, Alizarin Red and Oil Red O staining were applied, respectively.

The other half of the litter was stained with Alizarin red by the technique of Staples and Schnell for skeletal examination (STAPLES; SCHNELL, 1964; KIM et al.

Cultures were then stained with alizarin red to assess matrix mineralization.

Biological characteristics of the cells were then examined using MTT assays, AKP activity assays, Alizarin Red S staining, and western-blotting, respectively.

Osteogenic differentiation ability of isolated hAMMSC was confirmed by positive Alizarin red S staining.

Alizarin red, calcein and tetracycline are commonly used PFCs in bone histomorphometry, appearing as red, green and bluish green respectively under the fluorescent microscope.

Alizarin Red staining was also performed on 2-D hASCs (Figure 2, right) to determine the potential for osteogenic differentiation.