The cells were resuspended in cold HBSS and aliquoted
in 20000 cells per 16.
Pooled stock from fresh samples from healthy psittaciform birds was prepared, aliquoted
, and stored for use.
Blood was obtained from each pig by cardiac puncture and directly aliquoted
into 2-ml sterile vials, one aliquot used liquid sodium heparin (1,000 USP units/ml) as an anticoagulant to obtain the whole blood for lymphocyte tests, another allowed to clot for 4 h for serum preparation.
The remaining swab transport medium was filtered, and 100 [micro]L of filtrate was aliquoted
onto each of 3 tissue culture plates containing rhabdomyosarcoma, Vero, and 293T cell lines for virus isolation.
The crude antigen obtained was aliquoted
and stored at -70[degrees]C until use.
Pellet containing purified Anaplasma bodies was resuspended in TS-PI Buffer, aliquoted
and preserved under liquid nitrogen.
22[micro]m filter, aliquoted
and stored at -20[degrees]C until use.
the cell suspension (100 pL) into each well of two 96-well tissue culture plates and incubated them at 37[degrees]C.
Bicuculline was aliquoted
in distilled water and diluted (1000-fold) in Ringer's to 10 [micro]M
Eggs were aliquoted
with a pasteur pipette to different treatments and to a control dish of eggs without added sperm as fertilization controls.
Transfected DCs are matured, aliquoted
Serum samples were aliquoted
and kept at -20oC until analysed.
Serum or plasma samples were thawed and aliquoted
into single-use vials for DMF immunoassays.
One mL of the sperm sample was aliquoted
into three parts.
3 mL of plasma was aliquoted
from each sample and frozen at -20[degrees]C overnight (~22 hours), and the remaining plasma was stored on cells and analyzed after 1, 3, and 5 hours of storage at room temperature.