aliquot

The cells were resuspended in cold HBSS and aliquoted in 20000 cells per 16.

Pooled stock from fresh samples from healthy psittaciform birds was prepared, aliquoted, and stored for use.

Blood was obtained from each pig by cardiac puncture and directly aliquoted into 2-ml sterile vials, one aliquot used liquid sodium heparin (1,000 USP units/ml) as an anticoagulant to obtain the whole blood for lymphocyte tests, another allowed to clot for 4 h for serum preparation.

The remaining swab transport medium was filtered, and 100 [micro]L of filtrate was aliquoted onto each of 3 tissue culture plates containing rhabdomyosarcoma, Vero, and 293T cell lines for virus isolation.

The crude antigen obtained was aliquoted and stored at -70[degrees]C until use.

Pellet containing purified Anaplasma bodies was resuspended in TS-PI Buffer, aliquoted and preserved under liquid nitrogen.

22[micro]m filter, aliquoted and stored at -20[degrees]C until use.

We aliquoted the cell suspension (100 pL) into each well of two 96-well tissue culture plates and incubated them at 37[degrees]C.

Bicuculline was aliquoted in distilled water and diluted (1000-fold) in Ringer's to 10 [micro]M

Eggs were aliquoted with a pasteur pipette to different treatments and to a control dish of eggs without added sperm as fertilization controls.

Transfected DCs are matured, aliquoted and cryopreserved.

Serum samples were aliquoted and kept at -20oC until analysed.

Serum or plasma samples were thawed and aliquoted into single-use vials for DMF immunoassays.

One mL of the sperm sample was aliquoted into three parts.

3 mL of plasma was aliquoted from each sample and frozen at -20[degrees]C overnight (~22 hours), and the remaining plasma was stored on cells and analyzed after 1, 3, and 5 hours of storage at room temperature.