With our handwashing and canine skin and ear canal swabbing techniques, a larger surface area was sampled, especially on human hands, and the PCR was presumably able to detect low cell numbers within a large sample
aliquot. The culture technique we used for human hands appears to be inadequate for screening purposes; however, we do not know the number or density of viable yeast cells on human hands that may be required to nosocomially spread infection in a clinical setting.
The Power Processor is easily enhanced by adding modular components such as: Hematology Outlet, which sorts CBC, CBC/diff, and CBC/diff/retic samples, as well as other noncentrifuged samples; Intelligent Alliquotter, for transferring serum from a primary tube to
aliquot tubes for subsequent testing on multiple analyzers; CHEMXpress, which provides connections for up to two Synchron LX series chemistry analyzers, resulting in improved handling of critical reruns, flexible stat.
The indexes A, B, and C refer to the ESR spectra of each of the 3
aliquots measured in each individual.
Dissolve trypsin at a 1mg/ml concentration in double distilled water and freeze small
aliquots for use.
According to the company, using the liquid handler, the sample can be
aliquoted and then analyzed with results saved directly in the database, providing efficient operation with advanced security.
Pellets were resuspended in 1 mL of PBS and divided in two 475-[micro]L
aliquots. Then, 25 [micro]L of mAb (60.11, anti-gC1qR) was added to one
aliquot, to attain a 1:20 working dilution.
METHODS: Three months of data from a Level 2 and Level 3 neonatal intensive care unit (NICU) were used to determine the comparative cost for red cell
aliquot transfusions using an eight bag aliquot/transfer system or the syringe set system.
Every 30 minutes an
aliquot of cells was harvested, and RNA and DNA were isolated by standard procedures (RNAeasy kit and Blood DNA kit, Qiagen, Hilden, Germany).
Initially an
aliquot of VMS is taken, to which, suppressor is added to ensure the deposition is inhibited to the furthest possible extent (brightener intercept solution).
An
aliquot was withdrawn for measurement of protein (Smith et al.
The two methods available are "batch" and "
aliquot."
Feedings consisted of a per capita 25 mg
aliquot of a finely ground 1:1 mixture of rabbit chow and fish food flakes, i.e., the 1-tadpole treatment received a 25 mg
aliquot whereas the 10-tadpole treatment received a 250 mg
aliquot at each feeding.
* Detection of SARS-CoV RNA by RT-PCR confirmed by a second PCR assay, by using a second
aliquot of the specimen and a different set of PCR primers
A 0.6-ml
aliquot of the supernatant was removed and evaporated to dryness under a stream of nitrogen (Meyer N-evap); the residue was redissolved in 12 ml methanol:water (80:20 v/v); and ce ntrifuged as above.
