The serum samples from both dams showed positive titre of 1:80 (160 IU) in serum agglutination test and rapid agglutination when tested against Rose Bengal Plate Test (RBPT) antigen (Fig.8).
Sensitivity of local antigen: Serial tenfold dilutions (Undiluted, 10-1 to 10-6) of all the three antigens were prepared in Normal saline and each dilution was subjected to agglutination test with known positive and negative antisera to find out the minimum number of bacterial cells (best dilution) required to be used for visible agglutination.
[12] Culture which is gold standard along with latex agglutination test becomes an important diagnostic tool for interpretation of the aetiological agent.
In a study carried out in the south of Iran, there was 7.8% antibody positivity (titer[greater than or equal to]1:40) among people in the at-risk group for brucellosis with standard tube agglutination test (14).
The serum agglutination test (SAT) for Brucella species was carried out in the Changchun Prefecture Center for Disease Prevention and Control (Changchun, China); the titer was 1:100, and hence, infection by Brucella species was confirmed.
A serological test using the microscopic agglutination test (MAT) was conducted in the Institute of Medical Research with a cut-off titre for seropositivity of [greater than or equal to]1: 100.