The antibody screen test involves incubating the patient's plasma with commercially available RBCs, in the presence of low-ionic strength solutions (LISS) or polyethylene glycol (PEG), which are used to optimize agglutination
Test (LAT)-For rapid diagnosis of acute bacterial meningitis.
Repeat Brucella agglutination
titer at three months fell to 1:160 and turned negative after nine months.
After incubation, the tubes were centrifuged briefly and observed for agglutination
5%) positive cases in cattle and buffalo using Rose Bengal plate test and serum agglutination
test by Faqir (1991), who also reported the presence of brucellosis in cattle and buffalo in the same area after screening 680 animals and (8.
Vet, France) and Latex Agglutination
(Antec Diagnostic, UK).
For cell grouping (Table 1), one drop of 5% three times washed cell suspension was mixed with one drop of appropriate anti-sera, mixed well and read for agglutination
after 30 minutes keeping the tubes at 37 degrees centigrade in incubator.
(100%) was scored as 4+, 75% agglutination
as 3+, 50% agglutination
as 2+, and 25% agglutination
Patients with AIHA and warm RBC autoantibodies do not have the spontaneous agglutination
of RBCs but can still have significant hemolysis as the RBCs coated with autoantibodies are removed by the reticuloendothelial system, which also presents with signs and symptoms of anemia and hemolysis.
test (MAT) is the gold standard for diagnosis of leptospira infection in dogs (Chappel et al.
is a clumping reaction between specific antibodies and a particulate antigen, such as erythrocytes or bacterial cells suspension, and it is usually applied in the study of adaptive immune responses to evaluate the antibody production (Plumb and Areechon, 1990; Chen and Light, 1994; Yildirim et al.
In the blood bank, the result is expressed as the inverse of the highest serum dilution which causes macroscopically visible agglutination
Serological diagnosis is usually performed using a tube agglutination
test (TAT), a rapid slide agglutination
test, and a gel immunodiffusion test [2-4].
All cows in milk production on these farms were serologically tested, first by using slow agglutination
test with the addition of EDTA to the antigen, and then, if results were positive, by a commercial ELISA.
ABSTRACTObjectives: To determine the etiological agents by Latex Particle Agglutination
(LPA) and Polymerase Chain Reaction (PCR) in patients admitted with Cerebrospinal Fluid (CSF) culture negative bacterial meningitis.