Surface analysis data of the samples Sample [R.sub.a] [R.sub.ms] [R.sub.max] [R.sub.area] (nm) (nm) (nm) [([micro]m) square] Agarose
on 7.086 9.025 62.433 25.059 Pl.Lcoated slide Agarose
on glass 7.334 9.156 63.601 25.063 slide PLL coaled 0.869 1.572 20.488 25.005 slide Avicenna Journal of Medical Biotechnology, Vol.
binds at least 4 X [10.sup.-7] mol biotin per milliliter of settled resin.
The total amount or quantity of the 50 [micro]g genomic DNA was measured at [OD.sub.260nm] by the absorption of ultra-violet (UV) light by the ring structure of purines and pyrimidines and the quality of the extracted DNA was analyzed through agarose
gel electrophoresis (Fig.
Method for detection of specific RNAs in agarose
gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
Digestion is checked in Agarose
gel electrophoresis with 2[micro]l of digestion mixture and visualized under transilluminator.
sols were prepared by dispersing the agarose
powder (Product codes: A426-05, CAS No: 9012-36-6, J.
1: Electropherogram of ethidium bromide stained 2% agarose
gel showing the required bands with length of approximately 600 bp obtained after RT-PCR of RNA from sample 1 to 10, 200 bp DNA Step Ladder by Promega was used as reference
Visualization of such small bands is difficult on agarose
To immobilize the oligonucleotide probes on the inner surface of the Eppendorf cap, an activated agarose
film was prepared on the polycarbonate surface.
All RT-PCR products were analyzed by electrophoresis on 2% agarose
gels and visualized by staining with ethidium bromide.
Techniques mastered during the program include agarose
, polyacrylamide and protein gel electrophoresis, DNA sequencing, gene mapping, restriction enzyme cleavage of DNA, recombinant gene technology, cloning, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (rtPCR), Southern and Western blotting, analysis of restriction fragment polymorhisms (RFLP), analysis of variable number tandem repeats (VNTR's), DNA fingerprinting for paternity and forensics analysis, protein analysis and bioprocessing.
In the first step chromosomes are bound to protein agarose
using antibody recognizing a form of hyperacetylated histone as in the regular ChIP analysis.
Rabbit anti-phosphothreonin(495) (phospho-[Thr.sup.495]) eNOS, mouse anti-phosphotyrosine (PY99), rabbit anti-eNOS (NOS3, N-20), rabbit anti-p-Akt1(serine at codon 473), goat anti-human Akt, protein A agarose
, and secondary antibodies (both horseradish peroxidase-conjugated and fluorescein-conjugated) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
In a test performed by BIPHA Corporation, a subsidiary of Yoshitomi Pharmaceutical Industries in Japan and an Amersham customer, STREAMLINE SP adsorbents, a mixture of agarose
and crystalline quartz, proved remarkably reliable, lasting more than 1,000 cycles without a dip in performance.
UltraClean 15 utilizes a silica binding particle method to purify DNA fragments from TAE or TBE agarose