Both cytospin and adherent cells
were fixed with 4% paraformaldehyde (in FSW) at room temperature for 15 min.
This paper will discuss the main cost drivers of the manufacture of allogeneic mesenchymal adherent cells
, and the need for early process development to ensure the commercial viability of a product taking into account quality, quantity and cost.
It has also been observed that shear rate can affect cell capture rate; however, shear stress does not have much effect on adherent cells
within the physiological range.
Developed by Horacio Espinosa and John Kessler, the localized electroporation device (LEPD) can be applied to adherent cells
, which are grown on an artificial substrate as opposed to free-floating in a culture medium and are able to continue growing and differentiating.
Sponceram[R] Carrier Discs for Adherent Cell
Culture, Rev 103, ZELLWERK GmbH, Germany, 2008, pp 1-2.
The new method, which has been developed using Vera adherent cells
attached to Cytodex 1 microcarriers, allows a 20 percent media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately 4h.
3B) anoikis-resistant cells through matrigel-coated transwell chambers was approximately 50% greater than that of their adherent cell
Table 1 summarizes the average percent coefficient of variation (%CV) and %CV range obtained using the three methods to analyze 90 cellular samples from suspension and adherent cell
lines at multiple concentrations.
In order to use the cellexis technology, the use of so-called semi-adherent or adherent cell
lines is required, i.
a privately-held life sciences company commercializing products to advance the study of cell biology, stem cell research, biopharmaceutical production, and drug discovery, today announces the availability of the Celigo[TM] Adherent Cell
Cytometer in Japan.
8220;Our design objective was to combine the best of suspension cultivation with the advantages of adherent cell
Proprietary angled dispensing ensures compatibility with all dispense protocols from gentle treatment of loosely adherent cell
monolayers to rigorous or viscous reagent additions.
I am also enthused about our strong collaborations which will help Cyntellect to drive high value solutions based on our LEAP[TM] Cell Processing Workstation and Celigo[TM] Adherent Cell
The Cellaxess Elektra opens a new dimension in RNAi delivery, high-throughput screening and electroporation of primary adherent cell
types," said Dr.
Global Eukaryotic Microcarrier (GEM) technology provides an optically clear and non-autofluorescent, pipettable substrate for adherent cell