Both cytospin and
adherent cells were fixed with 4% paraformaldehyde (in FSW) at room temperature for 15 min.
This paper will discuss the main cost drivers of the manufacture of allogeneic mesenchymal
adherent cells, and the need for early process development to ensure the commercial viability of a product taking into account quality, quantity and cost.
It has also been observed that shear rate can affect cell capture rate; however, shear stress does not have much effect on
adherent cells within the physiological range.
Developed by Horacio Espinosa and John Kessler, the localized electroporation device (LEPD) can be applied to
adherent cells, which are grown on an artificial substrate as opposed to free-floating in a culture medium and are able to continue growing and differentiating.
Sponceram[R] Carrier Discs for
Adherent Cell Culture, Rev 103, ZELLWERK GmbH, Germany, 2008, pp 1-2.
The new method, which has been developed using Vera
adherent cells attached to Cytodex 1 microcarriers, allows a 20 percent media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately 4h.
3B) anoikis-resistant cells through matrigel-coated transwell chambers was approximately 50% greater than that of their
adherent cell counterparts.
Table 1 summarizes the average percent coefficient of variation (%CV) and %CV range obtained using the three methods to analyze 90 cellular samples from suspension and
adherent cell lines at multiple concentrations.
In order to use the cellexis technology, the use of so-called semi-adherent or
adherent cell lines is required, i.
a privately-held life sciences company commercializing products to advance the study of cell biology, stem cell research, biopharmaceutical production, and drug discovery, today announces the availability of the Celigo[TM]
Adherent Cell Cytometer in Japan.
8220;Our design objective was to combine the best of suspension cultivation with the advantages of
adherent cell cultivation.
Proprietary angled dispensing ensures compatibility with all dispense protocols from gentle treatment of loosely
adherent cell monolayers to rigorous or viscous reagent additions.
I am also enthused about our strong collaborations which will help Cyntellect to drive high value solutions based on our LEAP[TM] Cell Processing Workstation and Celigo[TM]
Adherent Cell Cytometer systems.
The Cellaxess Elektra opens a new dimension in RNAi delivery, high-throughput screening and electroporation of primary
adherent cell types," said Dr.
Global Eukaryotic Microcarrier (GEM) technology provides an optically clear and non-autofluorescent, pipettable substrate for
adherent cell lines.
