The plates were incubated at 30[degrees]C for 5 days for Actinomycetes
, and at 37[degrees]C for 24 hours for Eubacteria 12.
Keywords: Desert soil, Actinomycetes
, Secondary metabolites, Structure elucidation.
As shown in Table 1, it is clear the growth ability for the twenty-one of identified Actinomycete
strains and thirty-five of identified as Rhizobium on the Burk's N-free media.
In the experiments, applying BOF tended to increase bacterium, actinomycete
and fungus populations, but SF application increased actinomycetes
only, compared with those prior to the experiment (Table 7).
During the 3 sampling campaigns and based on colony morphology, growth characteristics, and macroscopic examination, a total of twenty-three actinomycete
strains were collected on all isolation media with 3 being isolated from Ml and 20 from TS18 (Table 1).
have been isolated from diverse natural resources.
Antagonistic activity of actinomycete
and Pseudomonas isolates against the most pathogenic Ralstonia solanacearum strain R15 (pathogenicity assay not shown) was tested according to Almoneafy's method .
Trueperella (Arcanobacterium) pyogenes is a gram-positive opportunistic bacterium, belonging to the group of actinomycetes
(QUINN et al., 1994).
Jiang and Mulla (2009) reported Streptomyces spinosa, the soil actinomycete
which exhibit elevated activity against phytophagous insects and insects affecting human and animal health.
Cultural characterization was done by inoculating all the actinomycete
cultures into YEME agar medium .
The current studies mainly focus on tapping marine sediments as a source for isolating and characterizing actinomycete
strains capable of producing alkaline amylase suitable for industrial production.
Williams, "Preservation of actinomycete
inoculum in frozen glycerol," Microbios Letters, vol.
Spores of potential actinomycete
strain were scrapped from starch casein agar and inoculated into 50 mL of inoculation medium in 250 mL conical flask and kept in rotary shaker at 120 rpm for 48 hours at 28[degrees]C.