Previous studies have reported that CAPZB is an essential element of the actin cytoskeleton whereby it binds to the barbed ends of actin filaments
and regulates their polymerization .
Nebulin regulates actin filament
lengths by a stabilization mechanism.
In the present study, a very thin layer of actin filament
along the bulge surface was detected.
Weeds, "Cofilin changes the twist of F-actin: implications for actin filament
dynamics and cellular function," The Journal of Cell Biology, vol.
It has been proposed that ABD2 can bind first to one F-actin filament, which in turn "activates"ABD1 and enables it to bind in a more ordered manner to another actin filament
with a higher affinity .
(36) Furthermore, the myosin head could consistently interact with actin without moving away from the actin filament
because it was firmly affixed to a large scanning probe thus preventing diffusion.
The basal ES present on both sides of the two adjacent Sertoli cell (SC) is typified by actin filament
bundles existing between the endoplasmic reticulum (ER, see black asterisks) and TJ strand (see black arrowheads).
Organization of actin filaments
in the axial rod of abalone sperm revealed by quick-freeze technique.
As Chara myosin has a long neck and the high duty ratio, it probably move processively with a long step size of about a half pitch of the actin filament
along the actin cable.
Mitchison, "Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament
nucleation," Science, vol.
For actin filament
detection, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich), and stained with fluorescein-conjugated phalloidin (Sigma-Aldrich) at 37[degrees]C for 30 minutes.
(7) Therefore, the axial repeat of the troponin molecules (~38 nm, 5.5 nm x 7) along the actin filament
does not coincide with the crossover repeat of the actin filament
(~36 nm, 5.5 nm x 6.5).
Drubin, "Cofilin promotes rapid actin filament
turnover in vivo," Nature, vol.
In the presence of [Ca.sup.2+], gelsolin severs and caps polymeric actin filaments
(F-actin) (21): after binding of domain G1 and partly domain G2 to the actin filament
(F-actin), gelsolin rapidly severs F-actin and then remains bound to the barbed end of one of the newly formed filaments, forming a stable cap, thus inhibiting addition of further monomers.