The nucleus and acrosome
were stained in blue and green with DAPI and FITC-PSA, respectively.
Chronic restraint stress induces sperm acrosome
reaction and changes in testicular tyrosine phosphorylated proteins in rats.
Effect of glycerol concentration, Equex STM(r) supplementation and liquid storage prior to freezing on the motility and acrosome
integrity of frozen-thawed epididymal alpaca (Vicugna pacos) sperm.
05) enhanced post-thaw buffalo bull semen in terms of sperm motility percentage, viability, acrosome
integrity and sperm functional integrity.
5% DM accompanied with 300 mg of vitamin C/kg DM had a significant increase of the proportion of DHA in spermatozoa, motility, HOS and percentage of sperm with normal acrosome
, which may have beneficial effects on fertility.
It was recently observed that the acrosome
of many sperm are damaged after vitrification (25).
Quorum-sensing molecules induce acrosome
loss and cell death in human spermatozoa.
Effect of buffering systems on post-thaw motion characteristics, plasma membrane integrity, and acrosome
morphology of buffalo spermatozoa.
Success of fertilization depends on various cellular characteristics of the spermatozoa such as membrane and acrosome
integrity, mitochondrial activity, and production of reactive oxygen species (ROS) (Lu & Wu 2005, Espinoza et al.
ROS are needed for capacitation, acrosome
reaction, and ultimately fertilization .
After these alterations, spermatozoa undergo acrosome
reaction, in which hydrolytic enzymes enabling spermatozoa to fertilize oocytes are released [3, 4].
To explain the absence of acrosome
in globozoospermia, Dam et al .
In mammals, physiological hypotonicity can facilitate the acrosome
reaction in the sperm through calcium increase and acrosome
swelling (Rossato et al.
The database included the seminal variables: concentration, morphology (head, mid piece and tail), membrane structural integrity (MSI), acrosome
integrity (ACRI), and membrane functional integrity (MFI); proximal cytoplasmic droplet, (PCD), distal cytoplasmic droplet (DCD), and total cytoplasmic droplets (TCD).
The supravital stain trypan-blue was used to distinguish live and dead spermatozoa while giemsa stain was used to evaluate the integrity of the acrosome