These are suitable conditions for sperm cell capacitation 10 and also to evaluate their ability to undergo acrosome
Reactive oxygen species requirements for bovine sperm capacitation and acrosome
CRISP2 protein that has been localized in the acrosome
and sperm tail and involved in sperm-egg fusion, is a candidate gene in men infertility; however, analysis of CRISP2 variations in asthenozoo- and/or teratozoospermia failed to find a significant association (41).
Stage Cell types Size ([micro]m) Immature (I) Spermatogonia 10.35 [+ or -]0.11 Sertoli cells 10.63 [+ or -]0.46 Development (II) Spermatogonia 8.96 [+ or -]0.14 Spermatocytes Mature (III) Spermatogonia Spermatocytes 7.73 [+ or -] 1.02 Spermatids 4.76 [+ or -] 0.14 acrosome
Spermatozoa 11.16 [+ or -]0.2 spine TABLE 3.
In comprehensive view, it appears that the bulls with pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 could be used as a marker for selecting higher sperm concentration, mass motility, live spermatozoa acrosome
integrity and membrane integrity.
Experiment 3: The procedure was the same as second experiment except that the sperm acrosome
already had been reacted with inomycine.
After their nucleus condenses and is covered by an acrosome
cap, elongated spermatids differentiate into spermatozoa, followed by their release into the lumen of the seminiferous tubule.
Adiponectin protein is abundant in the tail region of bull sperm, while AdipoR1 is localized mainly at the equatorial and acrosome
region and AdipoR2 is expressed primarily on the sperm head region and on the equatorial line.
Besides, deficiency of PICK1 would cause male infertility in patients and animals as PICK1 is essential for vesicle trafficking from the Golgi apparatus to the acrosome
in sperm cells .
For the assessment of the acrosome
, the fluorescence probe FITC-PSA was evaluated in the epifluorescence microscope at 400X magnification (BX-51, Olympus, Tokyo- Japan).
(2011) to count two hundred sperms with acrosome
Moreover, they play the necessary role in sperm capacitation, acrosome
reaction, and fertilization [18-21].
reaction in Chlamydia-positive and negative patients.
Results of the study, clearly indicate that osmotic stress primarily damaged the spermatozoa internal structures more severely (mitochondria, acrosome
and DNA) rather than plasma membrane.