101 consist of isolated bacteria from hospital surfaces and 28 consist of isolated bacteria from hands of staff and according to
Acidimetric test results respectively 73 species of Staphylococcus isolated from hospital surfaces and 19 species of Staphylococcus isolated from hands of staff was resistance to beta lactame antibiotics [15-17,20,21].
Sulfate by turbid metric method, Total dissolved solids were determined by heating at 180@C, chlorides by argintometic titration, alkalinity by
acidimetric titration with HCl, calcium and magnesium were determined by EDTA titration method whereas sodium and potassium by flame photometer (World Health Organization, 2004).