In order to perform more sophisticated ALMS experiments, including protein digestions, it is imperative to eliminate any excess anhydride so as not to inadvertently acetylate
amino acids becoming accessible as a result of processes like enzymatic digestions.
It is found to acetylate
H3 (lys14) strongly and H4 (lys 8 and 16) weakly in a free histone mixture and is found to be highly conserved throughout the eukaryotes (40).
Conversely, because CoA is unable to acetylate
the active-site cysteine residue, this amino acid is thus susceptible to chemical reaction in the presence of CoA.
Scientists have identified enzymes that acetylate
, deacetylate, phosphorylate, dephosphorylate, and methylate histones.
One is a fiber process and the other is a process to acetylate
wood of large dimensions using microwave technology.