To methanol (10 mL) was added sodium (30 mmol) and then thiourea (10 mmol) and acetoacetic acid
ethyl ester (10 mmol).
Reference Methods Sample  UPLC-LTQ Orbitrap-MS Serum  UPLC-QTOF-MS Serum  HPLC-ion trapTOF-MS Serum Reference Main findings  LysoPC(18:0), lysoPC(17:0), lysoPC(16:0), lysoPC(15:0), PA(20:4(5Z,8Z,11Z,14Z)e- 2:0), phenylalanyl phenylalanine, bilirubin glucuronide, acetoacetic acid
, L- threonine, and DHAP(18:0) showed significant differences between the survival group and nonsurvival group; lysoPC(14:0), phenylalanyl phenylalanine, and bilirubin glucuronide improved with treatment and had potential disease- monitoring capability.
There were 4 common metabolites, that is, phosphate acid, L-phenylalanine, acetoacetic acid
, and cystathionine ketimine, found in the serums of patients with CHD showing dampness syndrome and patients with CRF showing dampness syndrome.
The concentration of plasma glucose, non-esterified fatty acids (NEFA), [beta]-hydroxybutyric acid (BHBA) and acetoacetic acid
(ACAC) were assayed using an automatic analyzer (Olympus AU 600, Diamond Diagnostics, Tokyo, Japan).
9:20 AM ACETOACETIC ACID
IMPACT ON BACTERIAL BIOFILMS
Once released from fat stores, free fatty acids travel to the liver where again, due to lack of insulin and increased catabolic hormones, the oxidation pathway is switched to generate ketone bodies: beta-hydroxybutyric acid and acetoacetic acid
. (15) Acetoacetic acid
spontaneously forms into acetone which, being volatile, is excreted via the lungs and gives the distinctive "nail polish remover" odour to the breath of those with DKA.