For the PCR-OLA numeric readout, the blank absorbance
value was subtracted from the absorbance
of the samples and the ratio of the absorbance
for the WT reaction to that of the mutant reaction was calculated for both of the mutations separately in each sample.
For the EIA assay, the reflex is not necessary for specimens with an absorbance
The PCR-ONH assay also clearly distinguished the control mutant 63D allele and the control wild-type H63 allele, with an absorbance
ratio of 0.
are marked as [greater than or equal to] 3.
The four wavelengths were selected for different absorbances
of major hemoglobin variants and water to facilitate the simultaneous determination of total hemoglobin concentration and oxygen saturation (5).
MTX is quantified by measurement of the decrease in absorbance
at 340 nm that occurs when NADPH is converted to [NADP.
was measured on a microtiter plate reader (LP400; Sanofi Diagnostics Pasteur) at 450 nm.
For most QD samples in this study the measured absorbances
were similar to those shown in Fig.
Stop the reaction using stop buffer as soon as the plate has developed enough for absorbance
The final concentrations used for taking the absorbance
The nature of chemical species in water and their concentration can be determined only by photoeolorimetric optical absorbance
value after in analysed water were caused specific color reactions in as many water samples as chemical species of interest.
We set out to determine if the absorbance
(log transmittance) as measured by an integrating sphere spectrophotometer of coatings with absorbing, reflecting, or scattering pigments was linearly proportional to the layer thickness.
Normalization of the above absorbances
is carried out according to the following equations:
of samples were scanned (Beckman DU-800) from 350 to 550 run in 1-mL airblanked quartz cuvettes.
All other absorbances
reading were taken with reference to this ([[lambda].