Determination of serum iron, total iron-binding capacity
and percent transferrin saturation; approved standard.
The relationship between chemically measured total iron-binding capacity
concentrations and immunologically measured transferrin concentrations in human serum.
Immunological measurement of transferrin compared with chemical measurement of total iron-binding capacity
The determination of the total iron-binding capacity
One might thereby infer that total iron-binding capacity
tends to increase proportionately to serum iron in females as they progress through menopause.
It is intuitive that, using any cost-analysis system, the cost of the single-step UIBC will be less than the cost of the two-step assay using serum iron plus UIBC, total iron-binding capacity
, or transferrin.
Serum iron and total iron-binding capacity
were assayed with the Hitachi 747 automatic chemical analyzer (Hitachi), and ferritin was measured by the ACS:180 chemiluminescence assay (Chiron).
The common laboratory tests for evaluating iron status are serum iron, total iron-binding capacity
, transferrin saturation, and ferritin (1).
The assay of serum iron and total iron-binding capacity
(TIBC) has been recommended as the first step of a phenotypic diagnosis.
Transferrin saturation was calculated by dividing the serum iron by the total iron-binding capacity
and multiplying by 100.
If the binding of iron to TRF is uncomplicated, then a high degree of correlation between the two measurements is likely and the expected ratio of total iron-binding capacity
(TIBC) in [micro]mol/L to TRF in g/L should be 25.
Total iron-binding capacity
(TIBC) (1) indicates the total amount of iron to saturate plasma or serum transferrin, which is a binding protein for iron.