The basket shown above started with a large mother fern (Asplenium bulbiferum) on top and leatherleaf fern (Rumohra adiantiformis), piggy-back plant (Tolmiea menziesii
), and cyclamen on the sides.
Tolmiea menziesii contains both diploid (2n = 14) and tetrapoloid populations (2n = 28) (Soltis 1984), and both cytotypes were included herein.
The first group (Group 1), comprises species of Lithophragma, Tellima grandiflora, Bensoniella oregona, Tolmiea menziesii, all species of Heuchera examined, and Mitella pentandra, M.
To explore further the phylogenetic position of Tolmiea menziesii, we conducted a series of shorter phylogenetic analyses that involved all of those taxa in Group 1, and several different outgroups, all taken from within the remainder of the Heuchera group (all from within Group 2 of island 1).
Most of the size variation observed in ITS-1 and ITS-2 for members of the Heuchera group is the result of two relatively large deletions, as interpreted from outgroup comparisons: (1) a 12-bp deletion within ITS-1 of both populations of Conimitella williamsii; and (2) a 14-bp deletion in ITS-2 of both samples of Tolmiea menziesii. With the exception of these two deletions, very few of the ITS sites required gaps for sequence alignment.
The sister status of diploid and tetraploid Tolmiea menziesii is also well supported (decay value of 8).