To prepare gel based vaccine sterile aluminium hydroxide gel was addedat a final concentration of 10 % in the virus suspension having a titer of 104 TCID50
To investigate the patterns of HIV infection in the different MDM types, a total of 6 donors from two independent experiments were infected with 5,000 x TCID50
of HIV-1 pWTThaL or pUC19.
Mycoplasma mycoides suspension (15mg/100ml=0.1ml), 0.2ml of PPR virus with 104 units of TCID50
in monovalent, 0.02ml of PPR virus with 105 units of TCID50
in combined vaccines, 0.2ml of FMD O virus with 106 units of TCID50
in monovalent and 0.02ml of FMD O virus with 107 units of TCID50
,in combined vaccine Aluminium hydroxide gel (4%) and Thiomersal Sodium (0.05%) were added in each of the vaccines as an adjuvant and preservative, respectively.
The following LD were found for the different detection systems: [10.sup.-0.5] TCID50
m[L.sup.-1] for gD-SYBR, [10.sup.-1.5] TCID50
m[L.sup.-1] for gD-Plexor, [10.sup.-2.5] TCID50
m[L.sup.-1] for gB-Taq.
Table 1: Results of virus titration (TCID50
method) in McCoy cells Virus Number of Number of CPE+wells/ dilution inoculated positive total numbers wells Wells for of wells CPE [10.sup.-1] 4 4 1 [10.sup.-2] 4 4 1 [10.sup.-3] 4 4 1 [10.sup.-4] 4 4 1 [10.sup.-5] 4 3 0.75 [10.sup.-6] 4 0 0 [10.sup.-7] 4 0 0
The 50% tissue culture infective dose (TCID50
) per mL was calculated according to the method of Reed and Muench.
Residual viral infectivity in supernatants was assayed on cell lines for comparing the TCID50
to the inoculum titer.
Os diferentes cultivos celulares foram infectados com virus (25pl/ poco contendo 100 TCID50
) e, a seguir, incubados a 37[degrees]C com 5% de C[O.sub.2] durante duas horas, quando, entao, o inoculo foi retirado.
6 October 2010 - Australian pharmaceutical company Viralytics Ltd (ASX: VLA) said today that an independent Data Safety Monitoring Committee allowed the company to move to a dose of CAVATAK at 109 TCID50
which is 10 times higher than the dosing just completed in the ongoing Phase I study.
Each dose of 1.5 ml of sheep pox/goat pox vaccine contains a minimum of 102.5 TCID50
of freeze dried live attenuated virus from the reference strain KSGP O-240.
Dotted lines represent the limit of linear range of quantification for viral RNA (2.903 [log.sub.10] matrix gene copies/mL) (A) or the detection limit by TCID50
assay (1.789 [log.sub.10] [TCID5.sub.0] / mL) in MDCK cells (B).
The biological titer of 7th passage of the serotype on BHK-21 cell line was 106.4 mean tissue culture infective dose (TCID50
) per ml.
After 24 hours of incubation, this pre-formed MDBK cell monolayer was infected with 0.5mL of an [10.sup.7,5] [TCID.sub.50] [mL.sup.-1] suspension of BoHV-1 ([10.sup.7,2] TCID50
) per 150[cm.sup.2] flasks in 60mL of infection medium, without adsorption step.
In the second arm, patients will also receive, on days one through five of each 28-day cycle, intravenous REOLYSIN at a dose of 3x1010 TCID50
The median tissue culture infectious dose 50% (TCID50
) was determined by microtitration test as described by Frey and Liess (1971).Antibodies against BVDV were investigated using the serum neutralization test (SNT).