Cells were routinely grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS and antibiotics (100 mg/ml penicillin and 50 mg/ml streptomycin sulfate
) at 37[degrees]C with 5% C[O.sub.2] atmosphere.
Adult flies were kept in fine mesh screen cages (30 x 30 x 30 cm) and provided with a liquid diet containing water, sugar and brewer's hydrolyzed yeast (Tzanakakis 1971) with access also to solid diet containing hydrolyzed brewer's yeast, sugar, dried egg yolk powder and streptomycin sulfate
(Tsitsipis & Kontos 1983).
A factorial experimental design was implemented combining 3 egg densities (10, 50, and 100/mL) and 3 antibiotic treatments (control, no antibiotic; 5 mg/mL streptomycin sulfate
; 5 mg/mL tetracycline erythromycin 2.5:2.5 mg/mL).
26239-42, Nacalai Tesque, Kyoto, Japan) and 500 [micro]g/ml streptomycin sulfate
aureus than tetracycline hydrochloride, erythromycin, streptomycin sulfate
, polymyxin B sulfate or neomycin sulfate.