While the chemical nature of Pilobolus sporangiospore cell walls has not been reported, it is widely assumed that, as with other zygomycetes, they are comprised of some combination of chitin and/or chitosan.
2 M sorbitol which reduced cytolysis of sphaeroplasts, and Tween 20[R] to reduce sporangiospore clumping.
Microscopic techniques were used to determine whether or not sporangiospore cell walls were being degraded with the release of sphaeroplasts as a result of enzyme treatments.
Sporangia are dark brown, colu mellae are conical and sporangiospores
are striate and angular to ellipsoidal.
DNA was extracted from the sporangiospores
using techniques described previously (Foos et al.
are ovoid in shape and columella are subglbose.
Nature of septate septate hyphae Probable Aspergillus Aspergillus identity flavus niger Isolates 3 4 Colony Brownish White description colony becoming becoming grayish brown darker with ages Morphological Filamentous, filamentous characteristic foot cell, Stolon, rhizoids short Ovoid conidiophores sporangiospores
Nature of septate coenocytic hyphae Probable Aspergillus Rhizopus sp.
Fungal isolates were readily identified by their characteristic morphology such as shape, size, colony morphology, nature of conidiophores, sporangiospores
The isolated bacterial strains were identified by using standard biochemical tests and fungal strains were by macroscopic observation of fungal colonies and microscopic identification of conidia and sporangiospores
in Lactophenol Cotton Blue staining method (15,16).
Conidiospores are unprotected spores formed by mitosis at the tips of hyphae, and sporangiospores
are spores produced within a sac called a sporangium.
However, at room temperature, C recurvatus grows faster and produces hyphae, sporangiospores
and, occasionally, zygospores.
Spherules filled with round fungal endospores and scattered individual sporangiospores
of coccidioides were identified adjacent to areas of placental infarction (Figures 2 and 3).