In the study, two of five colonies in the FGF knock-in fibroblast were detected by southern blot analysis
Polymerase chain reaction (PCR) amplification and Southern blot analysis
are methods currently in widespread use for detection of clonal IGH rearrangements.
6 2 How can southern blot analysis
be used to distinguish the transgene from the endogenous T-bet gene?
All samples were previously characterized by PCR and/or Southern blot analysis
Southern blot analysis
was achieved using PCR and RTPCR products.
The Southern blot analysis
with HindIII (that cuts at one end of the coding sequence of the fusion Bt gene) and PstI (that does not cut inside the coding sequence of the T-DNA but cuts once in the transformation vector) showed only one hybridization band, meaning that the transgene had been integrated at only one site in the genome in all the transgenics produced through Agrobacterium-mediated transformation (data not shown).
In this study, we also compared methylation levels of exonic (FREE2 CpG units 1 and 2) and intronic (FREE2 CpG units 6-12) sequences, the FMR1 activation ratios determined with methylation-sensitive Southern blot analysis
of the NruI restriction site (within the CpG island), and the results of FMRP immunostaining of blood lymphocytes (28).
The independence of the lines was confirmed by Southern blot analysis
Southern blot analysis
by using the ampicillin resistance gene of pBluescript KS (+) (Stratagene, La Jolla, CA) as a probe indicated that a resistance gene was carried on the 50-kb plasmid.
Molecular demonstration of EBV nucleic acids includes ISH methods and other methods in which the DNA is first extracted from the tissue prior to hybridization (ie, dot-blot methods, Southern blot analysis
, and PCR).
Polymerase Chain Reaction and Southern Blot Analysis
FMR1 Southern blot analysis
is used both to characterize samples with numbers of CGG repeats too large to amplify by the PCR and to determine the methylation status of the gene (11).
However, most multiplex techniques produce amounts and quality of DNA insufficient for Southern blot analysis
and only suitable for PCR (Dilworth and Frey, 2000; Karakousis and Langridge, 2003; Lange et al.
3,4,6,7] Intravascular lymphomatosis was originally believed to be a malignant proliferation of endothelial cells; however, further studies, including immunohistochemistry, Southern blot analysis
, and polymerase chain reaction gene rearrangement, have identified a B-cell lineage in the majority of cases.