A 96 well microplate (Nunc-Immunoplate-F96 Maxisorp, Denmark) was coated with 100 p.1 of Russell's viper venom
as antigen (1 [mu]g/l 00 [mu]l/well) diluted in phosphate buffered saline (pH 7.4) and kept overnight at 4[degrees]C.
(1,2) These findings consisted of a prolonged activated partial thromboplastin time (APTT), abnormal 1:1 mixing studies that did not correct into the normal range of the laboratory reagent/instrument combination, falsely decreased coagulation factors that corrected upon dilution, a positive dilute Russell's Viper venom
assay, and a positive platelet neutralization assay.
could neutralize actions induced by Russell's Viper venom
(Alam et al., 1996).
ABBREVIATIONS: APL = antiphospholipid antibodies; APS = antiphospholipid syndrome; APTT = activated partial thromboplastin time; [[beta].sub.2]-GPI = [beta.sub.2]-glycoprotein I; dRVVT = dilute Russell's viper venom
time; ELISA = enzyme-linked immunosorbent assay; Ig = immunoglobulin; KCT = kaolin clotting time; LA = lupus anticoagulant.
Use of a simplified dilute Russell's viper venom
time (DRVVT) confirms heterogeneity among "lupus anticoagulants".
Q Do you have a reference or a procedure for the dilute Russell's viper venom