methylation levels of blood samples at four CpG sites in ASPA, PDE4C,
from genes: ADAR, AQP11, ITGA2B, and PDE4C. Although quadratic
genomic regions: ELOVL2, ASPA, PDE4C, FHL2, CCDC102B, C1orf132, and
Further, several CpGs were associated with genes related with life expectancy, such as with PDE4C
. However, in the Lothian Birth Cohort 1936 (LBC1936), where there are lower number of deaths, 99 CpG model could not assess CpG correlation with mortality, while age predictions models by Hannum et al.
Whole cell lysate proteins were then extracted and evaluated by immunoblotting for PDE4 isoforms (PDE4A, PDE4B, PDE4C, and PDE4D) with [beta]-actin utilized as a loading control.
Also consistent with such a mechanism, we observed that PDE4C was significantly decreased when the cells were pretreated with indomethacin, while, by contrast, PDE4A and PDE4C were significantly increased when the cells were pretreated with PG[E.sub.2].