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Database analysis revealed that the primary structure of protein encoded in ORF1 and ORF2 showed high similarity with aldehyde dehydrogenase and iron containing alcohol dehydrogenase of P.
These two sets of primers were designed to produce 343 bp segment of ORF1 region (21).
ORF will continue to broadcast both ORF1 and ORF2 also in standard definition.
A full-length L1 element is approximately 7 kb long and contains a 5'-ntranslated region (UTR) with an internal promoter, two open reading frames (ORF1, ORF2), and a 3'-UTR terminated by a polyadenylated tail.
In the field of television, the Germans have put a lot of products on the market because they allow private ownership, while Austria has made do with the government-owned ORF1 and ORF2.
Yet, ORF1 and ORF2 are more than holding up their end in the face of all that competition.
Phylogenetic trees of the nucleotide and amino acid sequences of ORF1, ORF2, and ORF3 of HEV strains showed that LCK-3110 is most closely related to the Vietnam-105 strain (Figure 4; online Technical Appendix Figure 3, panels A, B), sharing 93.7% nt identity.
In total, 4 open reading frames (ORFs) have been identified: ORF1 at the 5' end encodes nonstructural protein NS1; ORF2 which is in the middle of the genome encodes nonstructural protein NP1, a unique Bocavirus characteristic structure that is absent in most other members of Parvoviridae [5]; overlapping ORF3 and ORF4 encodes structural proteins VP1 and VP2, respectively [5].
PCV2 has three major ORFs; ORF1 encodes two replication-related proteins, Rep and Rep', which are essential for viral DNA replication [29]; ORF2 encodes the major capsid protein ofPCV2; and ORF3 encodes the nonstructural ORF3 protein [30].
El analisis de la secuencia mostro un extremo 5' terminal no codificante (UTR), en el cual se encontro un segmento parcialmente complementario al ARN ribosomal 18S, y a continuacion una primera pauta abierta de lectura (ORF1) que se solapa en 33 nt con el extremo 5' de un segundo ORF (ORF2; Figura 2), tal como se habia descrito previamente (Espinha et al., 2004).
However, LINE-1 proteins (ORF1 and ORF2) can be found in human somatic tissues (Ergun et al.
The genome of TTV exhibits an astonishingly high genetic diversity and is classified into nearly 39 genotypes incorporating three-hypervariable region within ORF1 (6,7) [12,13].