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ORF1 of JS1208 and BJSMQ also differed from that of GII.
TTV-DNA was detected using two nested-PCR procedures specific for the detection of sequences included in the ORF1 region (N22 PCR) (outer primers: 1901-1918/2227-2210; inner primers: 1919-1936/2192-2175) and in the UTR region (UTR PCR) (primers NG133/NG147; NG134/NG132) (19) of the virus.
2000b) performed by cloned from UTRPCR products representing the full-length TTV genomes obtained from sera of humans and non-human primates that showed common genomic organization with two open reading frame (ORFs), designated ORF1 and ORF2 and their sequences were determined.
Complete or near full-length open reading frame (ORF) 2 (viral protein [VP] 1) and partial ORF1 (RNA-dependent RNA polymerase [RdRp]) sequences were further obtained from 11 randomly selected Taiwan GII.
LINE-1 elements are approximately 6,000 bp long and contain two transcribable open reading frames (ORFs), ORF1 (an RNA-binding protein) and ORF2 (an endonuclease/reverse transcriptase).
2 degrees East orbital position is scheduled for December 5, and follows the successful roll-out of ORF1 HD in June 2008.
2-kb, including partial ORF1 [822 nt], full-length ORF2 [1621 nt], and ORF3 [866 nt]) for 3 representative GII.
The new channel will start broadcasting first of May and offer the complete program of ORF1 in HD format including the 31 matches of the UEFA EURO 2008[TM].
Recombination among different genotypes is frequently observed for human noroviruses, most commonly near the junction of ORF1 and ORF2, leading to a recommendation for multilocus genotyping (5).
ORF1 encodes a multifunctional nonstructural polyprotein with methyltransferase and RNA-dependent RNA polymerase genes often used for molecular typing.
Phylogenetic trees constructed using ORF1, ORF2, ORF3, and concatenated ORF1/ORF2, excluding the hypervariable region, showed that these 3 BcHEV isolates clustered with the 2 dromedary camel HEV7 strains and the HEV7 strain from the liver-transplant recipient with chronic hepatitis (Figure; online Technical Appendix Figure 1) (5,6).
Clinical samples were tested for MERS-CoV RNA by reverse transcription PCR (RTPCR) targeting the upE (upstream of E) and ORF1 (open reading frame 1) genes (6).